AB9738

Anti-14-3-3 γ Antibody

serum, Chemicon^®

• Código UNSPSC: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Propriedades

• fonte biológica: rabbit
• Nível de qualidade: 100
• forma do anticorpo: serum
• tipo de produto de anticorpo: primary antibodies
• clone: polyclonal
• reatividade de espécies: human, rat, sheep, chicken, mouse, bovine
• fabricante/nome comercial: Chemicon^®
• técnica(s): ELISA: suitable; immunohistochemistry: suitable; western blot: suitable
• nº de adesão NCBI: NM_012479.2
• nº de adesão UniProt: P61981
• Condições de expedição: wet ice
• modificação pós-traducional do alvo: unmodified
• Informações sobre genes: human ... YWHAG(7532)

Descrição

Descrição geral

Mammals express seven distinct 14-3-3 isoforms (gamma, epsilon, beta, zeta, sigma, theta, tau) that form multiple homo- and hetero- dimmers, thus rendering 14-3-3 as a key multifunctional regulatory molecule. 14-3-3 isomers have been implicated in several neurological disorders: for example isoforms gamma, epsilon and beta are expressed in the cerebrospinal fluid of Creutzfeldt-Jakob patients, but not patients suffering from other neurological conditions such as Alzheimer′s.

The 14-3-3 gamma antibody is one of a panel of antibodies that were generated against 14-3-3 isoforms and have demonstrated their potential for use as an ELISA-based diagnostic tool. These antibodies will allow the research to investigate the role of this protein in key cellular processes and neurological disorders such as Creutzfeldt-Jakob, Alzheimer′s and Parkinson′s Disease.

Especificidade

14-3-3 gamma. By Western blot the antibody recognizes the ~30 kDa protein.

Imunogênio

Synthetic peptide from the N-terminal area of sheep 14-3-3 gamma.

Aplicação

Anti-14-3-3 γ Antibody detects level of 14-3-3 γ & has been published & validated for use in ELISA, WB, IH.

Western blot: 1:6,000-1:12,000 using ECL.
Immunohistochemistry: 1:400-1:800 on mouse brain tissue which was treated by hydrated autoclaving at 121°C for 15 minutes prior to immunolabeling. Endogenous peroxidase was blocked with hydrogen peroxide in methanol and non-specific labeling with normal goat serum at 1:20 for 5 minutes.
ELISA
Optimal working dilutions must be determined by end user.

Informações legais

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Informações de segurança

• Código de classe de armazenamento: 10 - Combustible liquids