71340-M

pCDFDuet-1 DNA - Novagen

• Sinônimo(s): Duet-1 DNA, Novagen DNA Vector, pCDFDuet-1 DNA
• Código UNSPSC: 41106602
• NACRES: NA.51

Propriedades

• Formulário: liquid
• Nível de qualidade: 100
• fabricante/nome comercial: Novagen^®
• condição de armazenamento: OK to freeze
• Promotor: Promoter name: T7
• Seleção: spectinomycin
• temperatura de armazenamento: −70°C

Descrição

Descrição geral

pCDFDuet-1 is designed for the coexpression of two target genes. The vector encodes two multiple cloning sites (MCS) each of which is preceded by a T7 promoter, lac operator, and ribosome binding site (rbs). The vector also carries the pCloDF13 replicon, lacI gene and streptomycin/spectinomycin resistance marker. This vector can be transformed into the same cell with pETDuet-1, pACYCDuet-1, and pRSFDuet-1 or pCOLADuet-1 for the coexpression of up to eight target genes. ORFs inserted into MCS-1 can be sequenced using the ACYCDuetUP1 Primer and DuetDOWN1 Primer. ORFs inserted into MCS-2 can be sequenced using the DuetUP2 Primer and T7 Terminator Primer.

Aplicação

pCDFDuet-1 DNA has been used as a vector for the expression of recombinant proteins in E-coli cells.[1][2][3]

Advertência

Toxicity: Standard Handling (A)

Informações legais

NOVAGEN is a registered trademark of Merck KGaA, Darmstadt, Germany

Informações de segurança

• Código de classe de armazenamento: 10 - Combustible liquids
• Classe de risco de água (WGK): WGK 1
• Ponto de fulgor (°F): Not applicable
• Ponto de fulgor (°C): Not applicable