529625

Polyphosphoinositide-Binding Peptide, PBP10

A Rhodamine-B-labeled decapeptide derived from the (PIP₂)-binding region in segment-2 of gelsolin.

• Sinônimo(s): Polyphosphoinositide-Binding Peptide, PBP10, RhoB-GS _160-169, Rhodamine B-QRLFQVKGRR-OH, RhoB-GS_160-169, Rhodamine B-QRLFQVKGRR-OH
• Fórmula empírica (Notação de Hill): C₈₄H₁₂₇N₂₄O₁₅
• Número CAS: 794466-45-8
• Peso molecular: 1713.06
• Código UNSPSC: 12352211
• NACRES: NA.77

Propriedades

• Nível de qualidade: 100
• Ensaio: ≥95% (HPLC)
• forma: lyophilized solid
• fabricante/nome comercial: Calbiochem^®
• condição de armazenamento: OK to freeze; desiccated (hygroscopic); protect from light
• cor: pink-red
• solubilidade: DMSO: 2 mg/mL
• fluorescência: λ_ex 565 nm; λ_em 590 nm
• Condições de expedição: ambient
• temperatura de armazenamento: −20°C

Descrição

Descrição geral

A Rhodamine-B-labeled decapeptide derived from the (PIP₂)-binding region in segment-2 of gelsolin. Acts as a cell-permeable, reversible, and non-toxic agent that transiently disrupts actin filaments, which are essential for cell morphology and cell motility (~1-10 µM). Specifically binds to polyphosphoinositide (PPI) with high affinity and activates platelet without significantly affecting intracellular Ca²⁺ influx. Undergoes a reduction in fluorescence intensity upon binding to phosphatidylinositol 4,5-bisphosphate (PIP₂) and lysophosphatidic acid; the fluorescence is not affected in the presence of phosphatidylserine and phosphatidylcholine. Useful fluorescent probe for the cellular localization and stabilization of PPIs.

A Rhodamine-B-labeled decapeptide derived from the (PIP₂)-binding region in segment-2 of gelsolin. Acts as a cell-permeable, reversible, and non-toxic agent that transiently disrupts actin filaments, which are essential for cell morphology and cell motility (~1-10 µM). Specifically binds to polyphosphoinositide (PPI) with high affinity and activates platelets without significantly affecting intracellular Ca²⁺ influx. Undergoes a reduction in fluorescence intensity upon binding to phosphatidylinositol 4,5-bisphosphate (PIP₂) and lysophosphatidic acid; the fluorescence is not affected in the presence of phosphatidylserine and phosphatidylcholine. Useful fluorescent probe for the cellular localization and stabilization of PPIs. Excitation max.: ~565 nm; emission max.: ~590 nm.

Ações bioquímicas/fisiológicas

Cell permeable: yes

Primary Target
Specifically binds to polyphosphoinositide (PPI)

Product does not compete with ATP.

Reversible: yes

Embalagem

Packaged under inert gas

Advertência

Toxicity: Standard Handling (A)

Sequência

Rho-B-Gln-Arg-Leu-Phe-Gln-Val-Lys-Gly-Arg-Arg-OH

forma física

Supplied as a trifluoroacetate salt.

Reconstituição

Following reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 6 months at -20°C.

Outras notas

Bucki, R., et al. 2001. Biochemistry40, 15752
Cunningham, C.C., et al. 2001. J. Biol. Chem.276, 43390.

Informações legais

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Informações de segurança

• Código de classe de armazenamento: 11 - Combustible Solids
• Classe de risco de água (WGK): WGK 1
• Ponto de fulgor (°F): Not applicable
• Ponto de fulgor (°C): Not applicable