496260

Oncostatin M, Human, Recombinant, E. coli

• Sinônimo(s): Oncostatin M, Human, Recombinant, E. coli
• Número CAS: 106956-32-5
• Código UNSPSC: 12352202
• NACRES: NA.77

Propriedades

• recombinante: expressed in E. coli
• Nível de qualidade: 100
• Ensaio: ≥97% (SDS-PAGE)
• Formulário: lyophilized
• fabricante/nome comercial: Calbiochem^®
• condição de armazenamento: OK to freeze
• Impurezas: ≤1 EU/μg Endotoxin (EU/μg rhOSM)
• Condições de expedição: ambient
• temperatura de armazenamento: −70°C

Descrição

Descrição geral

Recombinant, human oncostatin M expressed in E. coli. Pleiotropic cytokine which inhibits growth of A375 melanoma cells and mouse M1 myeloid leukemic cells. Enhances the growth of both endothelial and hematopoietic cells. Has also been reported to have growth stimulatory activities on normal fibroblasts, AIDS-Kaposis sarcoma cells, and a human erythroleukemic cell line, TF-1.

Recombinant, human oncostatin M expressed in E. coli. Pleiotropic cytokine which inhibits growth of A375 melanoma cells and mouse M1 myeloid leukemic cells. Has also been reported to have growth stimulatory activities on normal fibroblasts, AIDS-Kaposi′s sarcoma cells, and human erythroleukemia cell line, TF-1.

Ações bioquímicas/fisiológicas

ED₅₀ = 0.15-0.30 ng/ml as measured in a cell proliferation assay using a factor-dependent human erythroleukemic cell line, TF-1

Advertência

Toxicity: Standard Handling (A)

forma física

Lyophilized from sterile-filtered 30% acetonitrile, 0.1% TFA, 50 µg BSA/µg rhOSM.

Reconstituição

Following reconstitution, aliquot and freeze (-70°C). Avoid freeze/thaw cycles of solutions. Stock solutions are stable for up to 3 months at -70°C.

Reconstitute to a final concentration of ≥10 µg/ml in sterile PBS containing ≥0.1% BSA or HSA.

Outras notas

Boileau, C., et al. 2000. J. Immunol.164, 5713.
Miyajima, A., et al. 2000. Cytokine Growth Factor Rev.11, 177.
Linsley, P.S., et al. 1990. Mol. Cell. Biol.10, 1882.
Kitamura, T., et al. 1989. J. Cell Physiol.140, 323.

Informações legais

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Informações de segurança

• Código de classe de armazenamento: 11 - Combustible Solids
• Classe de risco de água (WGK): WGK 3