400053

Hygromycin B, Streptomyces sp., Sterile-Filtered Solution in 25 mM HEPES, Cell Culture-Tested

Hygromycin B is an aminoglycoside antibiotic that inhibits the growth of prokaryotic and eukaryotic microorganisms and mammalian cells. Inhibits protein synthesis by interfering with the translocation of the 70S ribosome and causes misreading of mRNA.

• Sinônimo(s): Hygromycin B, Streptomyces sp., Sterile-Filtered Solution in 25 mM HEPES, Cell Culture-Tested
• Fórmula empírica (Notação de Hill): C₂₀H₃₇N₃O₁₃
• Número CAS: 31282-04-9
• Peso molecular: 527.52
• Número MDL: MFCD06795479
• Código UNSPSC: 12352200

Propriedades

• Nível de qualidade: 100
• Ensaio: ≥90% (HPLC)
• Formulário: liquid
• fabricante/nome comercial: Calbiochem^®
• condição de armazenamento: OK to freeze
• Condições de expedição: ambient
• temperatura de armazenamento: 2-8°C
• InChI: 1S/C20H37N3O13/c1-23-7-2-5(21)9(26)15(10(7)27)33-19-17-16(11(28)8(4-25)32-19)35-20(36-17)18(31)13(30)12(29)14(34-20)6(22)3-24/h5-19,23-31H,2-4,21-22H2,1H3/t5-,6+,7+,8-,9+,10-,11+,12-,13-,14-,15-,16+,17+,18-,19+,20?/m1/s1
• chave InChI: GRRNUXAQVGOGFE-KPBUCVLVSA-N

Descrição

Descrição geral

Hygromycin B is an aminoglycoside antibiotic, inhibits the growth of prokaryotic and eukaryotic microorganisms and mammalian cells. Inhibits protein synthesis by interfering with the translocation of the 70S ribosome and causes misreading of mRNA. Resistance to hygromycin B is conferred by a gene whose product, hygromycin B phospho-transferase that phosphorylates and inactivates the drug. Hygromycin B has been used to select mutants carrying the hygromycin B resistance gene in a wide variety of cells including bacterial, protozoal, yeast, fungal, plant, and mammalian cells. Typical concentrations used for mammalian cell selection are between 0.05 to 1 mg/ml. The appropriate concentration for use must be determined empirically for each cell type.

Advertência

Toxicity: Highly Toxic & Carcinogenic / Teratogenic (I)

forma física

50 mg/ml active antibiotic in sterile-filtered 25 mM HEPES, pH 7.2.

Outras notas

Due to the nature of the Hazardous Materials in this shipment, additional shipping charges may be applied to your order. Certain sizes may be exempt from the additional hazardous materials shipping charges. Please contact your local sales office for more information regarding these charges.

Sugimoto, K., et al. 1994. Plant J.5, 863.
Lee, M.G., and van der Ploeg, L.H. 1991. Gene105, 255.
Salauze, D., et al. 1990. Antimicrob. Agents Chemother.34, 1915.
Cullen, D., et al. 1987. Gene57, 21.
Santerre, R.F., et al. 1984. Gene30, 147.
Rao, S.N., et al. 1983. Antimicrob. Agents Chemother.24, 689.
Gonzalez, A., et al. 1978. Biochim. Biophys. Acta521, 459.

Informações legais

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Informações de segurança

• Pictogramas: GHS06
• Palavra indicadora: Danger
• Frases de perigo: H301,H312,H330
• Declarações de precaução: P260 - P264 - P270 - P280 - P302 + P352 + P312 - P304 + P340 + P310
• Classificações de perigo: Acute Tox. 1 Inhalation - Acute Tox. 3 Oral - Acute Tox. 4 Dermal
• Código de classe de armazenamento: 6.1A - Combustible acute toxic Cat. 1 and 2 / very toxic hazardous materials
• Classe de risco de água (WGK): WGK 3