About This Item
Produtos recomendados
técnica(s)
western blot: suitable
método de detecção
chemiluminescent
Descrição geral
Stripping and re-probing of Western blots offers several advantages:
1) Conservation of samples that are expensive or available only in limited quantities,
2) Analysis of a given blot using several different antibodies, e.g. subtype- or isoform-specific antibodies,
3) Re-analysis of anomalous results and confirmation with the same or a different antibody,
4) Correcting errors in incubation with the wrong antibody,
5) Cost savings in reagents and time by reusing the same blot.
While antigen and antibody-based immunoaffinity matrices, such as Sepharose conjugates, have been reused many times without compromising antigen-antibody reactivity, the need for pH extremes and chaotropic agents has precluded the application of these methods to Western blotting.
The MILLIPORE Re-Blot Western Blot Recycling Kit contains specially formulated solutions that quickly and effectively remove antibodies from Western blots without significantly affecting the immobilized proteins.
Advantages of the Re-Blot Western Blot Recycling Kit include:
·*No pungent-smelling b-mercaptoethanol is contained in the Antibody Stripping Solution.
·*Antibody stripping is done at room temperature. No heating of blots is required.
·*Blots can be stripped of antibodies in approximately 10 minutes at room temperature.
·*Reblocking of blots may be avoided in most instances.
·*Blots may be reused in less than 15 minutes.
Aplicação
The Re-Blot Western Blot Recycling Kit should be used only for qualitative purposes until it has been established by comparative blot analysis that stripping does not quantitatively affect a given antigen.
This product is for research use only; not for diagnostic or in vivo use.
Componentes
Armazenamento e estabilidade
Exoneração de responsabilidade
Código de classe de armazenamento
10 - Combustible liquids
Certificados de análise (COA)
Busque Certificados de análise (COA) digitando o Número do Lote do produto. Os números de lote e remessa podem ser encontrados no rótulo de um produto após a palavra “Lot” ou “Batch”.
Já possui este produto?
Encontre a documentação dos produtos que você adquiriu recentemente na biblioteca de documentos.
Nossa equipe de cientistas tem experiência em todas as áreas de pesquisa, incluindo Life Sciences, ciência de materiais, síntese química, cromatografia, química analítica e muitas outras.
Entre em contato com a assistência técnica