17-321
Rap1 Activation Assay Kit
Non-radioactive Rap1 Activation Assay Kit that uses Ral GDS RBD, agarose (Catalog # 14-455) to precipitate Rap1-GTP from cell lysates & detection by a Rap1 specific antibody.
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About This Item
Código UNSPSC:
12161503
eCl@ss:
32161000
NACRES:
NA.84
Produtos recomendados
Nível de qualidade
fabricante/nome comercial
Upstate®
técnica(s)
activity assay: suitable
affinity binding assay: suitable
nº de adesão NCBI
nº de adesão UniProt
Condições de expedição
dry ice
Informações sobre genes
human ... RAP1GDS1(5910)
Embalagem
Kit capacity: 20-30 assays
Componentes
100X GTPγS, 10mM (Cat.# 20-176)
100X GDP, 100mM (Cat.# 20-177)
Rap1 Activation Lysis Buffer, 2X
Anti-Rap1
Rap1 Assay Reagent (Ral GDS-RBD, agarose) (Cat.# 14-455)
100X GDP, 100mM (Cat.# 20-177)
Rap1 Activation Lysis Buffer, 2X
Anti-Rap1
Rap1 Assay Reagent (Ral GDS-RBD, agarose) (Cat.# 14-455)
Qualidade
Routinely evaluated by affinity precipitation assay. Ral GDS RBD, agarose precipitated GTP-Rap1 from HEK293 lysates. The precipitated GTP-Rap1 was detected by immunoblot analysis using Anti-Rap1.
Informações legais
UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany
Exoneração de responsabilidade
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Palavra indicadora
Warning
Frases de perigo
Declarações de precaução
Classificações de perigo
Eye Irrit. 2
Código de classe de armazenamento
10 - Combustible liquids
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The EMBO journal, 16(2), 252-259 (1997-01-15)
Rap1 is a small, Ras-like GTPase whose function and regulation are still largely unknown. We have developed a novel assay to monitor the active, GTP-bound form of Rap1 based on the differential affinity of Rap1GTP and Rap1GDP for the Rap
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