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Documentos Principais

126615

Sigma-Aldrich

Bovine Serum Albumin

10% Aqueous Solution, Nuclease-Free

Sinônimo(s):

Albumin, Bovine Serum, 10% Aqueous Solution, Nuclease-Free

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About This Item

Número CAS:
9048-46-8
Número MDL:
MFCD00198176
Código UNSPSC:
12352202
NACRES:
NA.25
Preço e disponibilidade não estão disponíveis no momento.

Nome do produto

Albumin, Bovine Serum, 10% Aqueous Solution, Nuclease-Free,

fonte biológica

bovine

Nível de qualidade

300

descrição

Merck USA index - 14, 8468

Formulário

liquid

fabricante/nome comercial

Calbiochem®

condição de armazenamento

OK to freeze

concentração

0.1 g/10 mL (Aqueous Solution)

técnica(s)

ELISA: suitable
PCR: suitable
immunoblotting: suitable

Impurezas

≤0.02% Fatty acids
≤10 ppm Heavy metal
≤2.0% Ash

atividade externa

Nuclease, none detected
Protease, none detected

Condições de expedição

ambient

temperatura de armazenamento

2-8°C

Categorias relacionadas

Descrição geral

Purified chromatographically to reduce DNases and RNases to exceptionally low levels.
Useful for applications in which acetylated BSA is not desirable, such as in antibody dilution, DNA footprinting and gel shift assay, ELISA, enzyme assay, enzyme stabilization, immunoblotting, immunofluorescence, PCR, restriction enzyme reactions, RIA, probe-based diagnostics, radioactive quenching, and receptor binding studies. Purified chromatographically to reduce DNases and RNases to exceptionally low levels.

Advertência

Toxicity: Standard Handling (A)

forma física

10% aqueous solution, 0.22 µm-filtered, pH 6.8-7.2.

Informações legais

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Código de classe de armazenamento

12 - Non Combustible Liquids

Classe de risco de água (WGK)

WGK 3

Ponto de fulgor (°F)

Not applicable

Ponto de fulgor (°C)

Not applicable

Certificados de análise (COA)

Busque Certificados de análise (COA) digitando o Número do Lote do produto. Os números de lote e remessa podem ser encontrados no rótulo de um produto após a palavra “Lot” ou “Batch”.

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Alan J Simmons et al.
STAR protocols, 3(3), 101570-101570 (2022-07-27)
In droplet-based single-cell RNA-sequencing (scRNA-seq) experiments, cells, along with some of their surrounding buffer and ambient material, are encapsulated into droplets for mRNA capture and barcoding. This protocol details the steps for human gut tissue dissociation using cold active protease
Larry E Tetone et al.
Proceedings of the National Academy of Sciences of the United States of America, 114(7), E1081-E1090 (2017-02-01)
The secondary channel (SC) of multisubunit RNA polymerases (RNAPs) allows access to the active site and is a nexus for the regulation of transcription. Multiple regulatory proteins bind in the SC and reprogram the catalytic activity of RNAP, but the
Timothy T Harden et al.
Nature communications, 11(1), 448-448 (2020-01-25)
RNA polymerases (RNAPs) transcribe genes through a cycle of recruitment to promoter DNA, initiation, elongation, and termination. After termination, RNAP is thought to initiate the next round of transcription by detaching from DNA and rebinding a new promoter. Here we
Larry J Friedman et al.
Proceedings of the National Academy of Sciences of the United States of America, 110(24), 9740-9745 (2013-05-31)
Sequence-specific DNA binding proteins must quickly bind target sequences, despite the enormously larger amount of nontarget DNA present in cells. RNA polymerases (or associated general transcription factors) are hypothesized to reach promoter sequences by facilitated diffusion (FD). In FD, a
Larry J Friedman et al.
Biophysical journal, 91(3), 1023-1031 (2006-05-16)
Complexes of macromolecules that transiently self-assemble, perform a particular function, and then dissociate are a recurring theme in biology. Such systems often have a large number of possible assembly/disassembly intermediates and complex, highly branched reaction pathways. Measuring the single-step kinetic
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Questions

1–2 of 2 Questions  

  1. Is the Bovine Serum Albumin solution (#126615) sterile? 

    1 answer

    1. Yes, this product is sterile.

      Helpful?

  2. In which buffer is the 10% aqueous solution, 0.22 μm-filtered, pH 6.8–7.2 prepared?

    1 answer

    1. This product is prepared in deionized, nuclease-free water.

      Helpful?

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