1.25184

Fractogel^® EMD SO₃⁻ (S) Resin

RoboColumn Fractogel^® SO3 (S), volume 0.6 mL, prepacked column

• Sinônimo(s): RoboCol Fractogel^® SO3 (S), 0.6ml
• Código UNSPSC: 41115711

Propriedades

• ligand: (Sulfoisobutyl)
• Nível de qualidade: 100
• Materiais: polypropylene housing
• linha de produto: Fractogel^®
• Formulário: prepacked column
• Parâmetros: ≤8 bar pressure; ~80 cm/hr max. flow rate
• Coluna: DI × C: 5 mm × 30 mm
• volume: 0.6 mL
• Grupo ativo da matriz: Methacrylate
• tamanho médio de partícula: 20-40 μm
• capacidade: ~150 mL, resin binding capacity
• aplicação(ões): recombinant protein; vaccine development
• temperatura de armazenamento: no temp limit

Descrição

Descrição geral

5 x 30 mm

Características e benefícios

RoboColumn Fractogel^® SO3 (S), 0.6ml enables:

• Efficient separation between monomer, dimer, and high molecular weight aggregates
• High binding capacity
• High resolution
• Efficient polishing step
• Compatibility with 2.5 % (v/v) aqueous benzyl alcohol containing 150 mM NaCl storage solution

Due to the titration behavior, the ion exchange capacity can be used from pH 2 to pH 12. The separation of proteins is based on reversible electrostatic interactions between the surface′s positively charged regions and the support. Proteins are retained efficiently on Fractogel^® EMD SO₃^- (S) when the pH of the buffer is about 1 unit below their isoelectric points (pl).

The strength of the binding depends on the following:

• the buffer system
• pH value of the buffer which determines the surface charge of the protein
• the concentration of the counter ions
• the charge density on the support (protein binding capacity)

Embalagem

Plastic bag(s)

Informações legais

FRACTOGEL is a registered trademark of Merck KGaA, Darmstadt, Germany