09-822

Anti-KLF5 (Krüppel-like factor 5) Antibody

serum, from rabbit

• Sinônimo(s): BTE-binding protein 2, Basic transcription element-binding protein 2, Colon krueppel-like factor, GC box binding protein 2, GC-box-binding protein 2, Intestinal-enriched krueppel-like factor, Kruppel-like factor 5, Kruppel-like factor 5 (intestinal), Tra
• Código UNSPSC: 12352203
• eCl@ss: 32160702
• NACRES: NA.41
• clone: polyclonal
• application: ChIP; WB
• reatividade de espécies: mouse
• técnica(s): ChIP: suitable; western blot: suitable
• citations: 3

Propriedades

• fonte biológica: rabbit
• forma do anticorpo: serum
• tipo de produto de anticorpo: primary antibodies
• clone: polyclonal
• reatividade de espécies: mouse
• técnica(s): ChIP: suitable; western blot: suitable
• nº de adesão NCBI: NP_033899
• nº de adesão UniProt: Q9Z0Z7
• Condições de expedição: wet ice
• modificação pós-traducional do alvo: unmodified
• Informações sobre genes: human ... KLF5(688)

Descrição

Descrição geral

Krüppel-like factors (KLFs) are zinc finger-containing transcription factors with regulatory functions in cell growth, proliferation, differentiation, and embryogenesis. KLF5, (also known as BTEB2 and IKLF), is essential for mouse embryo development. KLF5 activates many genes during cardiovascular remodeling, including PDGFa/b, Egr-1, PAI-1, iNOS, and VEGF receptors, making KLF a potential therapeutic target for cardiovascular disease. KLF5 also plays a role in angiogenesis and in development of several tumor types including breast, prostate, bladder, colon, esophagus, and skin. KLF5 is closely related to other of the KLF family members. However, KLF5 and KLF4 exhibit opposite effects on regulation of gene transcription, despite binding to similar, if not identical, cis-acting DNA sequences. In addition, KLF4 and 5 exert contrasting effects on cell proliferation in many instances; while KLF4 is an inhibitor of cell growth, KLF5 stimulates proliferation.

Especificidade

This antibody recognizes Krüppel-like factor 5 at the N-terminus.

Imunogênio

Epitope: N-terminus

Recombinant protein corresponding to mouse Krüppel-like factor 5 at the N-terminus.

Aplicação

Anti-KLF5 (Krüppel-like factor 5) Antibody is a Rabbit Polyclonal Antibody for detection of KLF5 (Krüppel-like factor 5) also known as Krüppel-like factor 5 & has been validated in WB.

Chromatin Immunoprecipitation Analysis (ChIP):
A representative lot was used by an independent laboratory in ChIP. (Ng Huck Hui, Genome Institute of Singapore)

Research Category
Epigenetics & Nuclear Function

Epigenetics & Nuclear Function

Research Sub Category
Transcription Factors

Pluripotent & Early Differentiation

Qualidade

Evaluated by Western Blot in mouse embryonic stem cell chromatin extract.

Western Blot Analysis: 1:2,000 dilution of this antibody detected Krüppel-like factor 5 on 10 µg of mouse embryonic stem cell chromatin extract.

Descrição-alvo

~55 kDa observed. An uncharacterized band appears at ~30 kDa

forma física

Rabbit polyclonal serum containing 0.05% sodium azide.

Unpurified

Armazenamento e estabilidade

Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

Nota de análise

Control
Mouse embryonic stem cell chromatin extract

Exoneração de responsabilidade

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Informações de segurança

• Código de classe de armazenamento: 10 - Combustible liquids
• Classe de risco de água (WGK): WGK 1