07-1500

Anti-G Protein Giα 1/2 Antibody

from rabbit

• Código UNSPSC: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Propriedades

• fonte biológica: rabbit
• Nível de qualidade: 100
• forma do anticorpo: purified antibody
• tipo de produto de anticorpo: primary antibodies
• clone: polyclonal
• reatividade de espécies: rat, human, bovine, mouse
• técnica(s): immunohistochemistry: suitable (paraffin); western blot: suitable
• Isotipo: IgG
• nº de adesão NCBI: NM_002069.5
• nº de adesão UniProt: P63096
• Condições de expedição: wet ice
• modificação pós-traducional do alvo: unmodified
• Informações sobre genes: bovine ... Gnai1(281790) ; human ... GNAI1(2770) ; mouse ... Gnai1(14677) ; rat ... Gnai1(25686)

Descrição

Descrição geral

GTP-binding proteins (G-proteins) play a critical role in signal transduction by coupling receptors to effectors. The intracellular consequences of G-protein activation include second messenger generation, protein phosphorylation, ion channel activation, gene induction, cell growth, and differentiation. Gi belongs to the pertussis toxin sensitive G-proteins. Gi consists of three different subtypes of alpha-subunits (alphai1, alphai2 and alphai3).

Especificidade

Recognizes G protein subunit αi1.

Imunogênio

Peptide corresponding to amino acids 345-354 of Gi1 and 346-355 of Gi2

Aplicação

Anti-G Protein Giα 1/2 Antibody is an antibody against G Protein Giα 1/2 for use in WB, IH(P).

Immunohistochemistry(paraffin):
Representative testing from a previous lot.
Optimal Staining of GI ALPHA 1/2 With Citrate pH 6.0 Epitope Retrieval: Rat Cerebellum GI ALPHA 1/2 representative staining pattern/morphology in rat cerebellum. Tissue was pretreated with citrate pH 6, antigen retrieval. A previous lot of this antibody was diluted to 1:100, using IHC-Select Detection with HRP-DAB.

Optimal working dilutions must be determined by end user.

Qualidade

Routinely evaluated by Western Blot on L6 lysates.

Western Blot Analysis: 1:500 dilution of this lot detected G protein Gialpha 1/2 on 10 μg of L6 lysates.

Descrição-alvo

~40 kDa

Ligação

Replaces: AB4067

forma física

Format: Purified

Armazenamento e estabilidade

Stable at -20°C in undiluted aliquots for up to 12 months from date of receipt.
Handling Recommendations: Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

Outras notas

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Informações de segurança

• Código de classe de armazenamento: 10 - Combustible liquids
• Classe de risco de água (WGK): WGK 2
• Ponto de fulgor (°F): Not applicable
• Ponto de fulgor (°C): Not applicable