06-1433

Anti-phospho-FGFR-1 (Tyr653/Tyr654) Antibody

from rabbit, purified by affinity chromatography

• Sinônimo(s): Basic fibroblast growth factor receptor 1, FGFR-1, bFGF-R-1, Fms-like tyrosine kinase 2, FLT-2, Proto-oncogene c-Fgr, CD331
• Código UNSPSC: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Propriedades

• fonte biológica: rabbit
• Nível de qualidade: 100
• forma do anticorpo: affinity isolated antibody
• tipo de produto de anticorpo: primary antibodies
• clone: polyclonal
• purificado por: affinity chromatography
• reatividade de espécies: human
• reatividade da espécie (prevista por homologia): Xenopus (based on 100% sequence homology), mouse (based on 100% sequence homology), zebrafish (based on 100% sequence homology), chicken (based on 100% sequence homology), bovine (based on 100% sequence homology), rat (based on 100% sequence homology)
• embalagem: antibody small pack of 25 μg
• técnica(s): cell based assay: suitable; western blot: suitable
• nº de adesão NCBI: NP_075598
• nº de adesão UniProt: P11362
• Condições de expedição: ambient
• modificação pós-traducional do alvo: phosphorylation (pTyr653/pTyr654)
• Informações sobre genes: human ... FGFR1(2260)

Descrição

Descrição geral

FGFR-1 (fibroblast growth factor receptor 1) is a member of the basic fibroblast growth factor family of proteins and is thought to be important in the induction and propagation of benign prostatic hyperplasia. A shortage of FGFR-1 causes death post-implantation and has been linked to neurofascin and neurite outgrowth. FGFR-1 expression has recently been found to be increased in patients with renal cell carcinoma and FGFR-1 may also have a role to play in early limb formation.

Especificidade

This antibody recognizes FGFR-1 phosphorylated at Tyr653/Tyr654.

Imunogênio

Epitope: Phosphorylated Tyr653/Tyr654

KLH-conjugated linear peptide corresponding to human FGFR-1 phosphorylated at Tyr653/Tyr654.

Aplicação

Detect phospho-FGFR-1 (Tyr653/Tyr654) using this Anti-phospho-FGFR-1 (Tyr653/Tyr654) Antibody validated for use in WB, Cell Function Assay.

Peptide Inhibition Assay Analysis: 0.2 µg/mL from a representative lot peptide blocked in HEK293T cells transfected with FGFR-1.

Research Category
Inflammation & Immunology

Research Sub Category
Cytokines & Cytokine Receptors

Qualidade

Evaluated by Western Blot in untreated and lambda phosphatase-treated HEK293T cells transfected with FGFR1.

Western Blot Analysis: 0.2 µg/mL of this antibody detected FGFR-1 in 10 µg of untreated and lambda phosphatase-treated HEK293T cells transfected with FGFR1.

Descrição-alvo

~145 kDa observed.
FGFR-1 has 19 isoforms and eight potential glycosylation sites that increase the expected size to 120 kDa for the immature form and 145 kDa for the mature form of the receptor (Hisaoka, K., et al. (2011). JBC. 286(24):21118-21128).

forma física

Affinity purified

Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Armazenamento e estabilidade

Stable for 1 year at 2-8°C from date of receipt.

Nota de análise

Control
Untreated and lambda phosphatase-treated HEK293T cells transfected with FGFR-1

Outras notas

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Exoneração de responsabilidade

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.