05-449-C
Anti-Bin1, clone 99D (Ascites Free) Antibody
clone 99D, 1 mg/mL, from mouse
Sinônimo(s):
Myc box-dependent-interacting protein 1, Amphiphysin II, Amphiphysin-like protein, Box-dependent myc-interacting protein 1, Bridging integrator 1, Bin1
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About This Item
Código UNSPSC:
12352203
eCl@ss:
32160702
NACRES:
NA.41
Produtos recomendados
fonte biológica
mouse
Nível de qualidade
forma do anticorpo
purified antibody
tipo de produto de anticorpo
primary antibodies
clone
99D, monoclonal
reatividade de espécies
mouse, chicken, human
concentração
1 mg/mL
técnica(s)
flow cytometry: suitable
immunofluorescence: suitable
immunohistochemistry: suitable
immunoprecipitation (IP): suitable
western blot: suitable
Isotipo
IgG2bκ
nº de adesão NCBI
nº de adesão UniProt
Condições de expedição
wet ice
modificação pós-traducional do alvo
unmodified
Informações sobre genes
chicken ... Bin1(424228)
human ... BIN1(274)
mouse ... Bin1(30948)
Categorias relacionadas
Descrição geral
Bin1, also known as Myc box-dependent-interacting protein 1, Amphiphysin II, Amphiphysin-like protein, Box-dependent myc-interacting protein 1, Bridging integrator 1, and encoded by the gene BIN1/AMPHL, is another protein involved in the regulation of synaptic vesicle endocytosis and interestingly also acts as tumor suppressor and inhibits malignant cell transformation. BIN1 is expressed in many tissues but has the highest expression in brain and muscle. BIN1 has isoforms and particular isoforms are expressed in particular tissues. For instance BIN1 isoform 11A is only expressed in the brain and found in axon initial segments and nodes of Ranvier. Mutations in BIN1 are associated with a disordered called centronuclear 2 myopathy. BIN1 is localized to nucleus but some isoforms are associated with cytoplasm.
Imunogênio
Linear peptide corresponding to human Bin1.
Aplicação
Immunohistochemistry Analysis: A 1:50 dilution from a representative lot detected Bin1 in human cerebellum and human skeletal muscle myocytes tissue.
Western Blotting Analysis: A representative lot detected Bin1 in primary chicken embryo fibroblasts (Telfer, J.F., et al. (2005). Cellular Signalling. 17:701-8).
Immunoprecipitation Analysis: A representative lot immunoprecipitated Bin1 in C2C12 cell lysate (Wechsler-Reya, R., et al. (1997). Cancer Res. 57:3258-63).
Western Blotting Analysis: A representative lot detected Bin1 in human and rodent cell lines (Wechsler-Reya, R., et al. (1997). Cancer Res. 57:3258-63).
Immunoprecipitation Analysis: A representative lot immunoprecipitated Bin1 in co-immunoprecipitation studies (Sakamuro, D., et al. (1996). Nat. Genet. 14:69-77).
Immunohistochemistry Analysis: A representative lot detected Bin1 in breast and liver carcinoma tissues (Sakamuro, D., et al. (1996). Nat. Genet. 14:69-77).
Immunoprecipitation Analysis: A representative lot immunoprecipitated Bin1 in C2C12 cell lysate (Wechsler-Reya, R. J., et al. (1998). Mol Cell Biol. 18:566-75).
Western Blotting Analysis: A representative lot detected Bin1 in C2C12 cell lysate (Wechsler-Reya, R. J., et al. (1998). Mol Cell Biol. 18:566-75).
Flow Cytometry Analysis: A representative lot detected Bin1 in C2C12 cell lysate (Wechsler-Reya, R. J., et al. (1998). Mol Cell Biol. 18:566-75).
Immunofluorescence Analysis: A representative lot detected Bin1 in C2C12 cells (Wechsler-Reya, R. J., et al. (1998). Mol Cell Biol. 18:566-75).
Western Blotting Analysis: A representative lot detected Bin1 in primary chicken embryo fibroblasts (Telfer, J.F., et al. (2005). Cellular Signalling. 17:701-8).
Immunoprecipitation Analysis: A representative lot immunoprecipitated Bin1 in C2C12 cell lysate (Wechsler-Reya, R., et al. (1997). Cancer Res. 57:3258-63).
Western Blotting Analysis: A representative lot detected Bin1 in human and rodent cell lines (Wechsler-Reya, R., et al. (1997). Cancer Res. 57:3258-63).
Immunoprecipitation Analysis: A representative lot immunoprecipitated Bin1 in co-immunoprecipitation studies (Sakamuro, D., et al. (1996). Nat. Genet. 14:69-77).
Immunohistochemistry Analysis: A representative lot detected Bin1 in breast and liver carcinoma tissues (Sakamuro, D., et al. (1996). Nat. Genet. 14:69-77).
Immunoprecipitation Analysis: A representative lot immunoprecipitated Bin1 in C2C12 cell lysate (Wechsler-Reya, R. J., et al. (1998). Mol Cell Biol. 18:566-75).
Western Blotting Analysis: A representative lot detected Bin1 in C2C12 cell lysate (Wechsler-Reya, R. J., et al. (1998). Mol Cell Biol. 18:566-75).
Flow Cytometry Analysis: A representative lot detected Bin1 in C2C12 cell lysate (Wechsler-Reya, R. J., et al. (1998). Mol Cell Biol. 18:566-75).
Immunofluorescence Analysis: A representative lot detected Bin1 in C2C12 cells (Wechsler-Reya, R. J., et al. (1998). Mol Cell Biol. 18:566-75).
Research Category
Apoptosis & Cancer
Apoptosis & Cancer
Research Sub Category
Apoptosis - Additional
Apoptosis - Additional
This Anti-Bin1, clone 99D (Ascites Free) Antibody is validated for use in Western Blotting and Immunohistochemistry and Immunoprecipitation and Flow Cytometry and Immunofluorescence for the detection of Bin1.
Qualidade
Evaluated by Western Blotting in human skeletal muscle tissue lysate.
Western Blotting Analysis: 1.0 µg/mL of this antibody detected Bin1 in 10 µg of human skeletal muscle tissue lysate.
Western Blotting Analysis: 1.0 µg/mL of this antibody detected Bin1 in 10 µg of human skeletal muscle tissue lysate.
Descrição-alvo
~60 kDa observed
forma física
Format: Purified
Protein G Purified
Purified mouse monoclonal IgG2bκ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Armazenamento e estabilidade
Stable for 1 year at 2-8°C from date of receipt.
Exoneração de responsabilidade
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Código de classe de armazenamento
12 - Non Combustible Liquids
Classe de risco de água (WGK)
WGK 1
Ponto de fulgor (°F)
Not applicable
Ponto de fulgor (°C)
Not applicable
Certificados de análise (COA)
Busque Certificados de análise (COA) digitando o Número do Lote do produto. Os números de lote e remessa podem ser encontrados no rótulo de um produto após a palavra “Lot” ou “Batch”.
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