330002S

E06 mAb-biotinylated

E06 Mouse Monoclonal Antibody (IgM), Biotinylated Anti-(Oxidized Phospholipid), 1X TBS (Tris Buffered Saline) with 1%BSA

• Sinônimo(s): E06 mAb-biotinylated, T15 antibody
• Código UNSPSC: 12352203
• NACRES: NA.45

Propriedades

• forma do anticorpo: purified from hybridoma cell culture
• tipo de produto de anticorpo: primary antibodies
• Ensaio: >95%
• Formulário: solution
• embalagem: pkg of 1 × 0.125 mL (polypropylene tube with screw cap (330002S-25ug))
• fabricante/nome comercial: Avanti Research^™ - A Croda Brand 330002S
• concentração: 0.2 mg/mL (330002S-25ug)
• Condições de expedição: dry ice
• temperatura de armazenamento: −70°C

Descrição

Descrição geral

Avanti now provides high purity E06 from an animal-free culture of hybridoma cells. The E06 antibody was developed by Dr. Joseph Witztum at the University of California, San Diego using apolipoprotein E knock-out mice fed a high-fat diet for seven months.

Aplicação

E06 mAb-biotinylated has been used in immunodetection.[1] It may be used in immunohistochemistry,[2] western blot analysis and in in vivo imaging with magnetic resonance imaging (MRI) techniques.

The E06-Biotin is primarily used for sandwich ELISA determination of ox-LDL in serum or plasma samples. It can also be used in direct ELISA, competitive (IHC) and Western Blot analysis. For use in direct ELISA, make a 4 μg/mL working solution of 12 μg E06-Biotin in 3 mL of 1% BSA in PBS (1:62.5 dilution). Make a serial (1:1) dilution of this working solution to titrate the antibody. For competitive ELISA make a 2.5 μg/mL working solution (1:100 dilution).

Ações bioquímicas/fisiológicas

The E06 antibody is a murine monoclonal IgM natural antibody that binds to oxidized phospholipid and reacts with oxidized Low-density lipoprotein (LDL),[3] oxidized High-density lipoprotein (HDL) and protein covalently modified by oxidized phospholipid. This antibody has been used to probe atherosclerotic plaque in rabbits, monkeys,[2] mice, zebrafish and humans. It also binds to apoptotic cells, but not viable cells.

Embalagem

0.5 mL Polypropylene Tube with Screw Cap (330002S-25ug)

Informações legais

Avanti Research is a trademark of Avanti Polar Lipids, LLC

Informações de segurança

• Código de classe de armazenamento: 10 - Combustible liquids
• Classe de risco de água (WGK): WGK 3
• Ponto de fulgor (°F): Not applicable
• Ponto de fulgor (°C): Not applicable