100600P
Avanti
E. coli Extract Polar
Avanti Research™ - A Croda Brand
Sinônimo(s):
E. coli Polar Lipid Extract
Faça loginpara ver os preços organizacionais e de contrato
About This Item
Produtos recomendados
Formulário
powder
embalagem
pkg of 1 × 1 g (100600P-1g)
pkg of 1 × 100 mg (100600P-100mg)
pkg of 5 × 100 mg (100600P-500mg)
fabricante/nome comercial
Avanti Research™ - A Croda Brand
tipo de lipídio
lipid extracts
Condições de expedição
dry ice
temperatura de armazenamento
−20°C
Descrição geral
This product is an extract of E. coli B (ATCC 11303) grown in Kornberg Minimal media at 37°C and taken at 3/4 log growth phase. Total E. coli lipid extract is a chloroform:methanol extract of the respective tissue. This extract is partitioned against deionized water and the chloroform phase is concentrated. Polar lipid extract is the total lipid extract precipitated with acetone and then extracted with diethyl ether.
Aplicação
E. coli Extract Polar has been used for the preparation of lipid standards for validation purposes. It has also been used for determining the molar percentages of lipids.
E. coli Extract Polar has been used:
- in liposome preparation for spectroscopy studies using fluorescent membrane dye, laurdan
- in multilamellar vesicles generation
- as standard lipid material for tandem mass spectrometry analysis
E. coli Extract Polar is suitable: to prepare lipid standard material by dissolving in methanol to prepare small unilamellar vesicle to prepare liposomes (in Tris-hydrochloric acid (HCl)
Ações bioquímicas/fisiológicas
E. coli Polar lipid extracts are useful for reconstituting membrane proteins. The phosphatidylethanolamine is the predominant lipid component of E. coli extract polar extract. The minor components include the phosphatidylglycerol (PG) and cardiolipin. These lipids are majorly derived from the leaflets of cytoplasmic membrane and inner leaflet of outer membrane. E. coli polar lipid extracts form bilayer by vesicle fusion.
Embalagem
5 mL Clear Glass Sealed Ampule (100600P-100mg)
5 mL Clear Glass Sealed Ampule (100600P-500mg)
60 mL Amber Wide Mouth Screw Cap Glass Bottle (100600P-1g)
Informações legais
Avanti Research is a trademark of Avanti Polar Lipids, LLC
geralmente comprado junto com este produto
Nº do produto
Descrição
Preços
Código de classe de armazenamento
11 - Combustible Solids
Escolha uma das versões mais recentes:
Certificados de análise (COA)
Lot/Batch Number
It looks like we've run into a problem, but you can still download Certificates of Analysis from our Documentos section.
Se precisar de ajuda, entre em contato Atendimento ao cliente
Já possui este produto?
Encontre a documentação dos produtos que você adquiriu recentemente na biblioteca de documentos.
Using supported bilayers to study the spatiotemporal organization of membrane-bound proteins
Methods in Cell Biology, 128, 223-241 (2015)
Molecular model for the solubilization of membranes into nanodisks by styrene maleic acid copolymers
Scheidelaar S, et al.
Biophysical Journal, 108(2), 279-290 (2015)
Assessing Membrane Fluidity and Visualizing Fluid Membrane Domains in Bacteria Using Fluorescent Membrane Dyes
Wenzel M, et al.
Bio-protocol, 8(20) (2018)
Assessing membrane fluidity and visualizing fluid membrane domains in bacteria using fluorescent membrane dyes
Wenzel M, et al.
Bio-protocol (2018)
Emily Peterson et al.
Biochimica et biophysica acta, 1808(2), 516-521 (2010-10-26)
Amantadine-sensitive proton uptake by liposomes is currently the preferred method of demonstrating M2 functionality after reconstitution, to validate structural determination with techniques such as solid-state NMR. With strong driving forces (two decades each of both [K(+)] gradient-induced membrane potential and
Nossa equipe de cientistas tem experiência em todas as áreas de pesquisa, incluindo Life Sciences, ciência de materiais, síntese química, cromatografia, química analítica e muitas outras.
Entre em contato com a assistência técnica