GC Analysis of Terpenes in Hops (Cascade Variety) on Equity-1 after Headspace SPME using 50/30 μm DVB/CAR/PDMS Fiber
Matériaux
Colonne analytique
Fibre SPME
Fibres SPME Divinylbenzene/Carboxen/Polydimethylsiloxane (DVB/CAR/PDMS)
needle size 23 ga, StableFlex, for use with autosamplerÀ utiliser avec
Flacons Headspace, haut à vis, fond arondi (flacon seul)
volume 10 mL, clear glass vial, thread for 18, O.D. × H 22.5 mm × 46 mm, pkg of 100 eaBouchon magnétique à visser sur flacons pour espace de tête, filetage de 18 mm
PTFE/silicone septum (white PTFE/blue silicone), septum thickness 1.5 mm, pkg of 100 eaÉtalon
CONDITIONS
sample/matrix
ground Cascade variety hop pellets
SPME fiber
Divinylbenzene/Carboxen/Polydimethylsiloxane (DVB/CAR/PDMS), 50/30 μm (57298-U)
extraction
20 min, headspace, 40 °C
desorption process
3 min, 270 °C
sample preparation
30 min equilibration at 40 °C prior to extraction
sample preparation
fiber postbake after extraction, 3 min at 270 °C
column
Equity-1, 60 m x 0.25 mm I.D., 0.25 μm (28047-U)
oven
60 °C (2 min), 5 °C/min to 275 °C (5 min)
inj. temp.
270 °C
detector
MSD
MSD interface
300 °C
scan range
full scan, m/z 50-500
carrier gas
helium, 1 mL/min constant flow
liner
0.75 mm ID, SPME
Description
Remarque sur l'analyse
Terpenes are contained in the essential oils derived from plants, and often impart characteristic aromas to the plant and/or oil. In this application, headspace-SPME combined with GC/MS was used to analyze some of the terpenes present in the Cascade variety of hops. Chromatographic separation was performed on an Equity-1 capillary GC column, and identification was done using retention indices and spectral library match. Analysis showed a characteristic terpene profile, with high levels of ß-myrcene, caryophyllene, and humulene present.
Autres remarques
add 0.5 gm ground hops to a 10 mL headspace vial
Informations légales
null