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Characterization of TolC efflux pump proteins from Pasteurella multocida.

Characterization of TolC efflux pump proteins from Pasteurella multocida.

Antimicrobial agents and chemotherapy (2008-08-30)

Tamas Hatfaludi, Keith Al-Hasani, Michelle Dunstone, John Boyce, Ben Adler

RÉSUMÉ

Two TolC homologs, PM0527 and PM1980, were identified for Pasteurella multocida. A pm0527 mutant displayed increased susceptibility to a range of chemicals, including rifampin (512-fold) and acridine orange (128-fold). A pm1980 mutant showed increased susceptibility to rifampin, ceftazidime, and vancomycin.

MATÉRIAUX

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Description du produit

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Cycloheximide, from microbial, ≥94% (TLC)

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Cycloheximide solution, Ready-Made Solution, microbial, 100 mg/mL in DMSO, Suitable for cell culture

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Ethidium bromide, BioReagent, for molecular biology, powder

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Deoxycholic acid, ≥98% (HPLC)

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Rifampicine, suitable for plant cell culture, BioReagent, ≥95% (HPLC), powder or crystals

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Acridine Orange solution, 2% in H₂O

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Ethidium bromide solution, for fluorescence, ~1% in H₂O

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Nalidixic acid, ≥98%

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Erythromycin, meets USP testing specifications

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Sulfathiazole

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