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T7024

Sigma-Aldrich

N,N,N′,N′-Tetramethylethylenediamine

BioReagent, for molecular biology, ≥99% (GC)

Synonyme(s) :

1,2-Bis(dimethylamino)ethane, TEMED, TMEDA

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25 ML
70,50 €
50 ML
126,00 €
100 ML
232,00 €

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25 ML
70,50 €
50 ML
126,00 €
100 ML
232,00 €

About This Item

Formule linéaire :
(CH3)2NCH2CH2N(CH3)2
Numéro CAS:
110-18-9
Poids moléculaire :
116.20
Beilstein:
1732991
Numéro CE :
203-744-6
Numéro MDL:
MFCD00008335
Code UNSPSC :
41105319
ID de substance PubChem :
24900242
Nomenclature NACRES :
NA.31
Qualité:
for molecular biology
Essai:
≥99% (GC)
pb:
120-122 °C (lit.)

70,50 €

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Devis pour commande en gros

Qualité

for molecular biology

Niveau de qualité

200

Gamme de produits

BioReagent

Essai

≥99% (GC)

Limite d'explosivité

9.08 %

Indice de réfraction

n20/D 1.4179 (lit.)

pb

120-122 °C (lit.)

Pf

−55 °C (lit.)

Densité

0.775 g/mL at 20 °C (lit.)

Activité étrangère

DNase, RNase, protease, none detected

Chaîne SMILES 

CN(C)CCN(C)C

InChI

1S/C6H16N2/c1-7(2)5-6-8(3)4/h5-6H2,1-4H3

Clé InChI

KWYHDKDOAIKMQN-UHFFFAOYSA-N

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Application

This product is designated as molecular biology grade and is suitable for polyacrylamide gel electrophoresis (PAGE). It has been analyzed for the absence of nucleases and proteases.
TEMED is a tertiary amine that is used to catalyze the formation of free radicals from ammonium persulfate or riboflavin. TEMED-generated free radicals cause the compounds acrylamide and bis-acrylamide to polymerize and form the gel matrix polyacrylamide. Because TEMED is functional only in its free base form, low pH conditions will inhibit polymerization.[1],[2],[3]
The potential contribution of TEMED to elevated background in silver stained SDS PAGE has been studied.[4] TEMED has been used in the coating of soft polymers, such as polyacrylamide, in capillaries for investigation by atomic force microscopy.[5] Microchips containing oligonucleotides and proteins immobilized within gel pads have been prepared using TEMED.[6]
The capillary isoelectric focusing of proteins with an isoelectric point (pI) > 12 has been investigated through the use of TEMED.[7] TEMED has been used in the HPLC of small organic molecules.[8]

Quantité

Suggested use is 35 μl for 50 ml acrylamide gel solution.

Pictogrammes

FlameSkull and crossbonesCorrosion
GHS02,GHS06,GHS05

Mention d'avertissement

Danger

Mentions de danger

H225,H302,H314,H331

Classification des risques

Acute Tox. 3 Inhalation - Acute Tox. 4 Oral - Eye Dam. 1 - Flam. Liq. 2 - Skin Corr. 1B

Code de la classe de stockage

3 - Flammable liquids

Classe de danger pour l'eau (WGK)

WGK 1

Point d'éclair (°F)

61.7 °F - closed cup

Point d'éclair (°C)

16.5 °C - closed cup

Équipement de protection individuelle

Faceshields, Gloves, Goggles, type ABEK (EN14387) respirator filter

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Certificats d'analyse (COA)

Lot/Batch Number
BCCM7546
BCCL1561
BCCK6320
BCCJ9691
BCCH5634

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Retrouvez la documentation relative aux produits que vous avez récemment achetés dans la Bibliothèque de documents.

Consulter la Bibliothèque de documents

A V Vasiliskov et al.
BioTechniques, 27(3), 592-594 (1999-09-18)
The manufacturing of microchips containing oligonucleotides and proteins immobilized within gel pads, ranging in size from 10 x 10 to 100 x 100 microns, is described. The microchips are produced by photo- or persulfate-induced copolymerization of unsaturated derivatives of biomolecules
Andrews, A. T.
Electrophoresis: Theory, Techniques, and Biochemical and Clinical Applications, 21-24 (1986)
G Patras et al.
Electrophoresis, 20(10), 2039-2045 (1999-08-18)
A novel experimental procedure to study the factors that contribute to background silver staining in sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE) is described. A mechanism is proposed for background silver staining, which was found to result primarily from the redox
Deepa Mohan et al.
Journal of chromatography. A, 979(1-2), 271-276 (2002-12-25)
The non-availability of commercial carrier ampholytes in the pH range greater than 11 has contributed to difficulties in focusing and resolving highly basic proteins/peptides using capillary isoelectric focusing (cIEF). Two different approaches, involving the use of N,N,N',N'-tetramethylethylenediamine (TEMED) and ampholyte
H Rosing et al.
Journal of chromatography. B, Biomedical applications, 668(1), 107-115 (1995-06-09)
A sensitive high-performance liquid chromatographic (HPLC) assay has been developed and validated for the quantitation of the novel anticancer agent topotecan and topotecan as the total of its lactone and carboxylate forms in human plasma. Linear response in analyte standard
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Questions

1–2 of 2 Questions  

  1. How can I determine the shelf life / expiration / retest date of this product?

    1 answer

    1. If this product has an expiration or retest date, it will be shown on the Certificate of Analysis (COA, CofA). If there is no retest or expiration date listed on the product's COA, we do not have suitable stability data to determine a shelf life. For these products, the only date on the COA will be the release date; a retest, expiration, or use-by-date will not be displayed.
      For all products, we recommend handling per defined conditions as printed in our product literature and website product descriptions. We recommend that products should be routinely inspected by customers to ensure they perform as expected.
      For products without retest or expiration dates, our standard warranty of 1 year from the date of shipment is applicable.
      For more information, please refer to the Product Dating Information document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/449/386/product-dating-information-mk.pdf

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  2. How is shipping temperature determined? And how is it related to the product storage temperature?

    1 answer

    1. Products may be shipped at a different temperature than the recommended long-term storage temperature. If the product quality is sensitive to short-term exposure to conditions other than the recommended long-term storage, it will be shipped on wet or dry-ice. If the product quality is NOT affected by short-term exposure to conditions other than the recommended long-term storage, it will be shipped at ambient temperature. As shipping routes are configured for minimum transit times, shipping at ambient temperature helps control shipping costs for our customers. For more information, please refer to the Storage and Transport Conditions document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/316/622/storage-transport-conditions-mk.pdf

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