PURE1A
Protein A Antibody Purification Kit
sufficient for 10 purifications
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About This Item
Code UNSPSC :
12352203
Nomenclature NACRES :
NA.32
Produits recommandés
Utilisation
sufficient for 10 purifications
Conditions d'expédition
wet ice
Température de stockage
2-8°C
Description générale
The Protein A Antibody Purification Kit provides all of the reagents necessary to isolate monoclonal and polyclonal antibodies.
Spécificité
Capacity/Run (by Species)
Human IgG 20-25 mg
Mouse IgG1 8-12 mg
Mouse IgG2a 10-15 mg
Mouse IgG2b 6-10 mg
Mouse IgG3 15-20 mg
Rabbit IgG 8-12 mg
Goat IgG 2-4 mg
Bovine IgG 8-12 mg
Human IgG 20-25 mg
Mouse IgG1 8-12 mg
Mouse IgG2a 10-15 mg
Mouse IgG2b 6-10 mg
Mouse IgG3 15-20 mg
Rabbit IgG 8-12 mg
Goat IgG 2-4 mg
Bovine IgG 8-12 mg
Application
PURE-1A offers Protein A technology in a prepackaged, easy to use kit form. With this kit, milligram quantities of IgG can be purified from serum, ascites, or cell culture supernatants.
Purification of IgG from other species may be possible, however, the researcher will have to determine the suitability of the kit for their application.
The Protein A Antibody Purification Kit is suitable for use with IgGs from human, mouse, rabbit, goat, and bovine species.
Caractéristiques et avantages
- High capacity - purify up to 8 mg of mouse IgG or 25 mg of human IgG per column run
- Specific - will only bind IgG
- Easy to use - antibody is eluted and desalted in a single step, ready to use
- Gentle - avoids prolonged exposure of the antibody to low pH
Principe
Using our cartridge system, antibodies elute as highly purified proteins at physiological pH. Protein A is a powerful tool for isolation of antibodies from mammalian hosts. Protein A exhibits a high degree of specificity for IgG and ensures an antibody preparation virtually free of IgA, IgM and non-immunoglobulin serum proteins such as albumin.
Composants de kit seuls
Réf. du produit
Description
- Binding Buffer 225 mL
- Desalting Cartridge 1 ea
- Elution Buffer 75 mL
- HEPES Buffer 225 mL
- 5 ml Syringe 1
- 10 ml Syringe 1
- Protein A Cartridge 1 mL
- Regeneration Buffer 75 mL
Afficher tout (8)
Produit(s) apparenté(s)
Mention d'avertissement
Danger
Mentions de danger
Conseils de prudence
Classification des risques
Acute Tox. 4 Oral - STOT SE 1
Code de la classe de stockage
6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects
Point d'éclair (°F)
Not applicable
Point d'éclair (°C)
Not applicable
Certificats d'analyse (COA)
Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".
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Protocoles
Antibody purification protocols yield preparations containing endogenous IgG alongside specific antibodies.
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