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P4780

Sigma-Aldrich

TWEEN® 80

suitable for cell culture, suitable for insect cell culture, viscous liquid

Synonyme(s) :

Mono-oléate de polyoxy-éthylènesorbitane, Mono-oléate de polyéthylène glycol sorbitane, Monooléate de POE (20)-sorbitane, Polysorbate 80

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100 ML
49,80 €
500 ML
56,30 €
1 GAL
259,00 €

49,80 €

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Sélectionner une taille de conditionnement

Changer de vue
100 ML
49,80 €
500 ML
56,30 €
1 GAL
259,00 €

About This Item

Numéro CAS:
9005-65-6
Numéro MDL:
MFCD00082107
Code UNSPSC :
12161700
Nomenclature NACRES :
NA.25

49,80 €

Veuillez contacter notre Service Clients pour connaître la disponibilité de ce produit.
Devis pour commande en gros

Description

non-ionic

Niveau de qualité

200

Forme

viscous liquid

Poids mol.

average mol wt ~1310

Composition

Oleic acid, ≥58.0% (balance primarily linoleic, palmitic, and stearic acids)

Technique(s)

cell culture | insect: suitable
cell culture | mammalian: suitable

CMC

0.012 mM

Densité

1.06 g/mL at 25 °C (lit.)
1.07 g/cm3 at 25 °C (lit.)

Chaîne SMILES 

CCCCCCCC/C=C/CCCCCCCC(=O)OCCOCC(C1C(CC(O1)OCCO)OCCO)OCCO

InChI

1S/C32H60O10/c1-2-3-4-5-6-7-8-9-10-11-12-13-14-15-16-17-30(36)40-25-24-37-27-29(39-22-19-34)32-28(38-21-18-33)26-31(42-32)41-23-20-35/h9-10,28-29,31-35H,2-8,11-27H2,1H3/b10-9+

Clé InChI

RGPBUVUVZKQNHD-MDZDMXLPSA-N

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Description générale

TWEEN 80 is a polyethylene sorbitol ester[1] and has a molecular weight of 1.31 kDa. It is useful as a stabilizer and emulsifier commercially. It is known to be used in ice-cream for its drying property.[2]

Application

Non-ionic detergent used for selective protein extraction and isolation of nuclei from mammalian cell lines.
TWEEN® 80 has been used:
  • in Listeria monocytogenes enumeration using filtration method[3]
  • as a hydrophilic emulsifier in the preparation of W1/O/W2 emulsion[4]
  • as a component of 7H9 plus oleic albumin dextrose catalase (OADC) in Mycobacterium Bovis bacillus Calmette–Guerin (BCG) Pasteur strain culturing[5]

Informations légales

TWEEN is a registered trademark of Croda International PLC

Code de la classe de stockage

10 - Combustible liquids

Classe de danger pour l'eau (WGK)

WGK 1

Point d'éclair (°F)

>235.4 °F - closed cup

Point d'éclair (°C)

> 113 °C - closed cup

Équipement de protection individuelle

Eyeshields, Gloves

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Certificats d'analyse (COA)

Lot/Batch Number
BCCK9226
BCCL8632
BCCL4140
BCCM3831
BCCL5402

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TWEEN® 80 protein grade detergent, 10% solution, sterile-filtered, for immunoprecipitation and for the solubilization of membrane-bound proteins

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Sigma-Aldrich

S6760

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SAFC

8.17061

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TWEEN® 80 FG

Sigma-Aldrich

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TWEEN® 20 viscous liquid, suitable for cell culture

Sigma-Aldrich

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USP

1547969

Polysorbate 80

Ice Cream, 86-86 (2003)
Low-fat Gouda cheese made from bovine milk-olive oil emulsion: physicochemical and sensory attributes
Felfoul I, et al.
Journal of Food Science and Technology, 52(10), 6749-6755 (2015)
A contribution to the improvement of Listeria monocytogenes enumeration in cold-smoked salmon
Besse NG, e al.
International Journal of Food Microbiology, 91(2), 119-127 (2004)
Gangotri Siddappa et al.
Molecular carcinogenesis, 56(11), 2446-2460 (2017-06-16)
Effective chemoprevention is critical for improving outcomes of oral cancer. As single agents, curcumin and metformin are reported to exhibit chemopreventive properties, in vitro as well as in patients with oral cancer. In this study, the chemopreventive efficacy of this
Differential requirements for L-citrulline and L-arginine during antimycobacterial macrophage activity
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Journal of Immunology, 195(7), 3293-3300 (2015)
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Questions

1–2 of 2 Questions  

  1. How is shipping temperature determined? And how is it related to the product storage temperature?

    1 answer

    1. Products may be shipped at a different temperature than the recommended long-term storage temperature. If the product quality is sensitive to short-term exposure to conditions other than the recommended long-term storage, it will be shipped on wet or dry-ice. If the product quality is NOT affected by short-term exposure to conditions other than the recommended long-term storage, it will be shipped at ambient temperature. As shipping routes are configured for minimum transit times, shipping at ambient temperature helps control shipping costs for our customers. For more information, please refer to the Storage and Transport Conditions document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/316/622/storage-transport-conditions-mk.pdf

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  2. How can I determine the shelf life / expiration / retest date of this product?

    1 answer

    1. If this product has an expiration or retest date, it will be shown on the Certificate of Analysis (COA, CofA). If there is no retest or expiration date listed on the product's COA, we do not have suitable stability data to determine a shelf life. For these products, the only date on the COA will be the release date; a retest, expiration, or use-by-date will not be displayed.
      For all products, we recommend handling per defined conditions as printed in our product literature and website product descriptions. We recommend that products should be routinely inspected by customers to ensure they perform as expected.
      For products without retest or expiration dates, our standard warranty of 1 year from the date of shipment is applicable.
      For more information, please refer to the Product Dating Information document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/449/386/product-dating-information-mk.pdf

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