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Key Documents

L2650

Sigma-Aldrich

Lectin from Glycine max (soybean)

peroxidase conjugate, lyophilized powder

Synonyme(s) :

SBA, Soybean agglutinin

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About This Item

Code UNSPSC :
12352202
Nomenclature NACRES :
NA.32

Source biologique

Glycine max (soybean)

Conjugué

peroxidase conjugate

Pureté

85.00-100.00% (Warburg-Christian)

Forme

lyophilized powder

Puissance

<80 μg per mL agglutination activity

peroxidase activity

25-100 units/mg protein

Composition

Protein, ~95% modified Warburg-Christian

Technique(s)

western blot: suitable

Numéro d'accès UniProt

P05046

Température de stockage

−20°C

Informations sur le gène

soybean ... LE1(100818710)

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Application

General Western Blot Protocol:
  • Glycoprotein sample size: 500ng
  • Lectin Concentration: 0.1ug/ml

  1. Load samples at 500 ng of glycoprotein per lane
  2. Run 4-20% Bis-Tris SDS page gel
  3. Transfer gel to a PVDF membrane
  4. Block membrane for 1 hr at RT with RIPA buffer (R0278 Sigma)
  5. Incubate HRP lectin at 0.1ug/ml with RIPA buffer for 2 hours at RT
  6. Wash membrane 5 x 5 minutes with 25ml RIPA buffer
  7. Detect using chemiluminescent substrate (CPS1-120)

Actions biochimiques/physiologiques

SBA is not blood group specific but has an affinity for N-acetyl-D-galactosamine.

Définition de l'unité

One unit will form 1 mg purpurogallin in 20 sec from pyrogallol at pH 6.0, at 20 °C.

Forme physique

Contains sodium citrate

Notes préparatoires

Conjugates are prepared from affinity purified lectin.
Prepared from peroxidase type VI (P8375) using a modification of the method of O′Sullivan, which favors low molecular weight conjugates. Repurified after conjugation by affinity chromatography.

Remarque sur l'analyse

Agglutination activity is expressed in μg/ml and is determined from serial dilutions in phosphate buffered saline, pH 6.8, of a 1 mg per mL solution. This activity is the lowest concentration to agglutinate a 2% suspension of human blood group A erythrocytes after 1 hr incubation at 25 °C.

Code de la classe de stockage

13 - Non Combustible Solids

Classe de danger pour l'eau (WGK)

WGK 3

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable

Équipement de protection individuelle

Eyeshields, Gloves, type N95 (US)

Certificats d'analyse (COA)

Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".

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Retrouvez la documentation relative aux produits que vous avez récemment achetés dans la Bibliothèque de documents.

Consulter la Bibliothèque de documents

C Biel et al.
Archives of toxicology, 96(6), 1815-1827 (2022-04-17)
Human Precision-cut intestinal slices (hPCIS) are used to study intestinal physiology, pathophysiology, drug efficacy, toxicology, kinetics, and metabolism. However, the use of this ex vivo model is restricted to approximately a 24 h timeframe because of declining viability of the hPCIS
Carin Biel et al.
Toxicology in vitro : an international journal published in association with BIBRA, 69, 104974-104974 (2020-08-24)
Precision-cut intestinal slices (PCIS) are used to study intestinal (patho)physiology, drug efficacy, toxicity, transport and metabolism ex vivo. One of the factors that limit the use of PCIS is a relatively short life-span. Moreover, culture-induced changes in cellular composition of
Jonathan Welch et al.
Regulatory toxicology and pharmacology : RTP, 125, 105022-105022 (2021-08-02)
The aim of the study was to use multiple in vitro assays to assess the effects of a model irritant, sodium dodecyl sulphate (SDS) (≤10 mM (0.29 %, w/v)), on an in vitro model of the airway, MucilAir™. The use of MucilAir™
Tomás Edreira et al.
eLife, 9 (2020-10-27)
In fission yeast, the septation initiation network (SIN) ensures temporal coordination between actomyosin ring (CAR) constriction with membrane ingression and septum synthesis. However, questions remain about CAR regulation under stress conditions. We show that Rgf1p (Rho1p GEF), participates in a
Daniel Sanchez-Guzman et al.
Scientific reports, 11(1), 6621-6621 (2021-03-25)
The human bronchial epithelium is the first line of defense against atmospheric particles, pollutants, and respiratory pathogens such as the novel SARS-CoV-2. The epithelial cells form a tight barrier and secrete proteins that are major components of the mucosal immune
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