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H5416

Sigma-Aldrich

FLT3 Ligand human

recombinant, expressed in HEK 293 cells, HumanKine®, suitable for cell culture

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About This Item

Numéro MDL:
MFCD01634859
Code UNSPSC :
12352202
Nomenclature NACRES :
NA.77

Source biologique

human

Niveau de qualité

100

Produit recombinant

expressed in HEK 293 cells

Pureté

≥95% (SDS-PAGE)

Forme

lyophilized powder

Puissance

≤0.8 ng/mL EC50

Qualité

endotoxin tested

Poids mol.

monomer 24-30 kDa (glycosylated)

Conditionnement

pkg of 10 μg

Conditions de stockage

avoid repeated freeze/thaw cycles

Technique(s)

cell culture | mammalian: suitable

Impuretés

≤1 EU/mg

Température de stockage

−20°C

Catégories apparentées

Description générale

Human FMS-like tyrosine kinase 3 (FLT3) ligand is a homodimer consisting of two short-chain α-helical bundles. The molecular weight of FLT3 ligand is 44 kDa.

Actions biochimiques/physiologiques

FLT3 ligand binds to the cell-bound tyrosine kinase receptor and activates them.
HumanKine FLT3 Ligand is expressed in human HEK 293 cells as a glycosylated monomer with an apparent molecular mass of 24-30 kDa. HumanKine FLT3 Ligand in human HEK 293 cells contributes to glycosylation.FLT3 ligand is a hematopoietic cytokine that regulates the proliferation of early hematopoietic cells. It has been shown to synergize with a variety of cytokines to stimulate the growth and differentiation of early hematopoietic cells. The FLT3 ligand also stimulates the expansion of monocytes and immature dendritic cells, and induces early B cell lineage differentiation and NK cell growth.

Forme physique

Lyophilized from a 0.2 μm filtered solution of 1x PBS.

Remarque sur l'analyse

The specific activity was determined by the dose-dependent stimulation of the proliferation of the human acute myeloid leukemia cell line OCI-AML5.

Informations légales

HumanKine is a registered trademark of Proteintech Group, Inc. and Humanzyme, Inc

Code de la classe de stockage

11 - Combustible Solids

Classe de danger pour l'eau (WGK)

WGK 3

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable

Certificats d'analyse (COA)

Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".

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Consulter la Bibliothèque de documents

Flt3 ligand structure and unexpected commonalities of helical bundles and cystine knots
Savvides S N, et al.
Nature Structural and Molecular Biology, 7(6), 486-486 (2000)
Nobuyuki Onai et al.
Annals of the New York Academy of Sciences, 1106, 253-261 (2007-03-16)
Flt3-ligand is a nonredundant cytokine in type I interferon-producing cell (IPC) and dendritic cell (DC) development. We demonstrated that IPC and DC differentiation potential is confined to Flt3(+)-hematopoietic progenitor cells, that Flt3-ligand drives development along both lymphoid and myeloid developmental
Structure-function analysis of FLT3 ligand-FLT3 receptor interactions using a rapid functional screen
Graddis T, et al.
The Journal of Biological Chemistry, 273(28), 17626-17633 (1998)
Chunaram Choudhary et al.
International journal of hematology, 82(2), 93-99 (2005-09-09)
Activating mutations of Fms-like tyrosine kinase 3 (Flt3) are the most common genetic lesions in acute myeloid leukemia (AML) and are present in approximately one third of AML patients. The 2 classes of Flt3 mutations are internal tandem duplications in

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