G1544

Anti-GFP, N-terminal antibody produced in rabbit

~1.0 mg/mL, affinity isolated antibody, buffered aqueous solution

• Synonyme(s) : Anti-Green Fluorescent Protein
• Numéro MDL: MFCD05665217
• Code UNSPSC : 12352203
• Nomenclature NACRES : NA.56

Propriétés

• Source biologique: rabbit
• Niveau de qualité: 200
• Conjugué: unconjugated
• Forme d'anticorps: affinity isolated antibody
• Type de produit anticorps: primary antibodies
• Clone: polyclonal
• Forme: buffered aqueous solution
• Concentration: ~1.0 mg/mL
• Technique(s): immunoprecipitation (IP): 1.0-2.5 μg using GFP fusion protein from transfected mammalian cell lysates; western blot: 0.25-0.5 μg/mL using 3-6 ng of purified GFP fusion proteins expressed in mammalian cell extracts
• Conditions d'expédition: dry ice
• Température de stockage: −20°C
• Modification post-traductionnelle de la cible: unmodified

Description

Description générale

Green fluorescent protein (GFP) chromophore contains hexapeptide fragment with Phe64-Ser-Tyr-Gly-Val-Gln69 aminoacid sequence. GFP structure contains a 11-stranded β-barrel and a central helix and corresponds to a molecular weight of 26 kDa.

Spécificité

Specific for GFP fusion proteins.

Immunogène

synthetic peptide corresponding amino acids 3-17 of GFP.

Application

Anti-GFP, N-terminal antibody produced in rabbit has been used:

• in immunoblotting
• in the histochemistry and immunohistochemistry
• in the immunoprecipitation

Actions biochimiques/physiologiques

Green fluorescent protein (GFP) has photochemical and diagnostic applications. It is also used widely in protein dynamics studies. The polyclonal anti-GFP, N-terminal rabbit antibody reacts specifically with GFP fusion proteins.

Forme physique

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 1% bovine serum albumin and 15 mM sodium azide.

Informations sur la sécurité

• Code de la classe de stockage: 10 - Combustible liquids
• Classe de danger pour l'eau (WGK): WGK 2
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable
• Équipement de protection individuelle: Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)