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F3008

Sigma-Aldrich

Anti-Mouse IgG (whole molecule)–FITC antibody produced in sheep

affinity isolated antibody, buffered aqueous solution

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About This Item

Numéro MDL:
Code UNSPSC :
12352203
Nomenclature NACRES :
NA.46

Source biologique

sheep

Conjugué

FITC conjugate

Forme d'anticorps

affinity isolated antibody

Type de produit anticorps

secondary antibodies

Clone

polyclonal

Forme

buffered aqueous solution

Technique(s)

direct immunofluorescence: 1:32

Température de stockage

2-8°C

Modification post-traductionnelle de la cible

unmodified

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Description générale

Immunoglobulin G (IgG) is available in large quantities in the human serum. It constitutes about 10-20% of the plasma proteins. IgG is a glycoprotein, with 82-96% proteins and 4-18% carbohydrates. It consists of four sub-classes i.e IgG1, IgG2, IgG3, and IgG4. IgG is composed of four polypeptide chains-two heavy chains (γ chains) and two light chains (κ or λ chains) which are linked by inter-chain disulfide bonds.

Application

Anti-Mouse IgG (whole molecule)−FITC antibody produced in sheep has been used in:
  • flow cytometry
  • immunocytochemistry
  • immunofluorescent staining

Actions biochimiques/physiologiques

IgG antibody subtype is the most abundant of serum immunoglobulins of the immune system. It is secreted by B cells and is found in blood and extracellular fluids and provides protection from infections caused by bacteria, fungi and viruses. Maternal IgG is transferred to fetus through the placenta that is vital for immune defense of the neonate against infections. The coupling of FITC to Anti- Mouse IgG (whole molecule) antibody allows for the visualization of protein by fluorescent microscopy.

Autres remarques

Antibody adsorbed with human serum proteins.

Forme physique

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 1% bovine serum albumin and 15 mM sodium azide.

Clause de non-responsabilité

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Code de la classe de stockage

10 - Combustible liquids

Classe de danger pour l'eau (WGK)

WGK 2

Point d'éclair (°F)

Not applicable

Point d'éclair (°C)

Not applicable


Certificats d'analyse (COA)

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M A Zimmermann et al.
Haemophilia : the official journal of the World Federation of Hemophilia, 20(3), e215-e221 (2014-03-08)
About 10% of mutations in haemophilia A cases generate a premature termination codon in the factor VIII gene (F8). Upon therapeutic FVIII substitution, it was noted that the risk of developing inhibitors is higher when the nonsense mutation is located
Mariella D'Alessandro et al.
Journal of cell science, 115(Pt 22), 4341-4351 (2002-10-12)
The intermediate filament cytoskeleton is thought to confer physical resilience on tissue cells, on the basis of extrapolations from the phenotype of cell fragility that results from mutations in skin keratins. There is a need for functional cell assays in
Katsushi Suzuki et al.
PloS one, 15(8), e0236781-e0236781 (2020-08-11)
It has been reported that Bach1-deficient mice show reduced tissue injuries in diverse disease models due to increased expression of heme oxygenase-1 (HO-1)that possesses an antioxidant function. In contrast, we found that Bach1 deficiency in mice exacerbated skeletal muscle injury
Masaki Sato et al.
Cancer research, 80(6), 1279-1292 (2020-01-11)
Pancreatic ductal adenocarcinoma (PDAC) is among the cancers with the poorest prognoses due to its highly malignant features. BTB and CNC homology 1 (BACH1) has been implicated in RAS-driven tumor formation. We focused on the role of BACH1 in PDAC
Alan Renton et al.
Carcinogenesis, 24(7), 1177-1182 (2003-06-17)
The p53 tumour suppressor gene is a transcription factor that can induce cell cycle arrest and apoptosis. In response to various stress-inducing signals, p53 level increases and this is accompanied with increased activities of p53. Interestingly, the methylxanthine caffeine can

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