293 GTP-AC-free, 05011003, NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA.
Source biologique
human kidney (embryonic)
Conditionnement
tube of 5 μg 05011003-DNA-5UG pkg of vial of cells 05011003-1VL
The human embryo kidney cell line 293 (Sigma Catalogue number. 85120602) adapted to grow in the animal component free medium Gene Therapy Medium 3 (Sigma G9916). This cell line grows in clumps in suspension. The original 293 cell line from which this cell line was derived was transformed with sheared human Ad5 DNA.
Gene Therapy Medium 3 (Sigma G9916) + 2mM Glutamine. When freezing cells down use 50:50 fresh culture medium:conditioned medium plus 10% DMSO.
Procédure de repiquage
Viability may be poor on resuscitation and may initially decrease further. Full recovery may take up to 2 weeks. A centrifugation step to remove the cryoprotectant is essential. Rapidly thaw the frozen ampoule in a water bath at 37°C for 1-2 minutes. Tran
Autres remarques
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