I accidentally bought this thinking it was MTT salt to use in a cell proliferation assay but it is MTT Formazan, the water insoluble end product! Therefore, it is useless for the assay. I suppose you could use it as a control to optimize your buffer for solubilizing the insoluble Formazan. The description for this product is misleading and should clarify that this is not what you need for an MTT assay, and refer the customer to MTT salt. That said, this is a nice purple crystalline powder and insoluble in water consistent with MTT Formazan, if that is what you actually want.
88417
MTT Formazan
MTT Formazan
MTT reduction end product, powder
Synonyme(s) :
Thiazolyl blue formazan
Se connecterpour consulter vos tarifs contractuels et ceux de votre entreprise/organisme
Sélectionner une taille de conditionnement
250 MG
59,10 €
1 G
173,00 €
59,10 €
Veuillez contacter notre Service Clients pour connaître la disponibilité de ce produit.
Sélectionner une taille de conditionnement
Changer de vue
250 MG
59,10 €
1 G
173,00 €
About This Item
Formule empirique (notation de Hill):
C18H17N5S
Numéro CAS:
Poids moléculaire :
335.43
Numéro MDL:
Code UNSPSC :
12352204
ID de substance PubChem :
Nomenclature NACRES :
NA.83
59,10 €
Veuillez contacter notre Service Clients pour connaître la disponibilité de ce produit.
Produits recommandés
Nom du produit
MTT Formazan, MTT reduction end product
Niveau de qualité
Forme
powder
Solubilité
chloroform: 10 mg/mL, clear, dark violet
Chaîne SMILES
Cc1nc(\N=N\C(=N\Nc2ccccc2)c3ccccc3)sc1C
InChI
1S/C18H17N5S/c1-13-14(2)24-18(19-13)23-22-17(15-9-5-3-6-10-15)21-20-16-11-7-4-8-12-16/h3-12,20H,1-2H3/b21-17+,23-22+
Clé InChI
GIFKVQOPAJDJLO-SZVLODPRSA-N
Vous recherchez des produits similaires ? Visite Guide de comparaison des produits
Actions biochimiques/physiologiques
MTT assay is a colorimetric reaction, generally used to examine cell viability. It involves the enzymatic reduction of a yellow-colored, water-soluble, 3-[4,5-dimethylthiazole-2-yl]-2,5-diphenyltetrazolium bromide (MTT) to purple-colored, water-insoluble, MTT-formazan. This reaction is catalyzed by mitochondrial dehydrogenase and only occurs in viable cells.[4][5] Thus, the amount of blue formazan is proportional to the number of viable cells. The absorbance of the formazan solution is analyzed spectrophotometrically at 570 nm.[6]
Autres remarques
Reviews: Tetrazolium salts and formazans[7]
Produit(s) apparenté(s)
Réf. du produit
Description
Tarif
Code de la classe de stockage
11 - Combustible Solids
Classe de danger pour l'eau (WGK)
WGK 3
Point d'éclair (°F)
Not applicable
Point d'éclair (°C)
Not applicable
Équipement de protection individuelle
Eyeshields, Gloves, type N95 (US)
Faites votre choix parmi les versions les plus récentes :
Déjà en possession de ce produit ?
Retrouvez la documentation relative aux produits que vous avez récemment achetés dans la Bibliothèque de documents.
Les clients ont également consulté
Polymeric nanoparticles for the selective therapy of inflammatory bowel disease
Test, 508, 377-397 (2012)
Primary cultures of cardiac myocytes as in vitro models for pharmacological and toxicological assessments
In Vitro Methods in Pharmaceutical Research, 209-223 (1997)
Chao Zhang et al.
Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie, 80, 253-259 (2016-05-03)
Costunolide, an active sesquiterpene lactone, is derived from many herbal medicines and it exhibits a broad spectrum of bioactivities such as anti-inflammatory, potential anti-tumor activity. Herein we assessed the anti-cancer effects of costunolide on U2OS cells and explored the underlying
Xi Jiang et al.
Medical science monitor : international medical journal of experimental and clinical research, 26, e924507-e924507 (2020-08-01)
BACKGROUND Curcumin derivative C086 (cur C086) is a potential chemotherapeutic agent for patients with osteosarcoma. In this study, the effects of cur C086 combined with cisplatin on the biological processes of osteosarcoma cells were investigated. MATERIAL AND METHODS In this
Jianing Li et al.
Pathology, research and practice, 215(10), 152591-152591 (2019-08-26)
The role of transforming growth factor beta (TGF-β) in lung cancer is well known. TGF-β-mediated cellular proliferation and angiogenesis through similar to mothers against decapentaplegic homolog 2 (Smad2) protein has also been well elucidated. Smad2 is a predicted target for
Active Filters
Notre équipe de scientifiques dispose d'une expérience dans tous les secteurs de la recherche, notamment en sciences de la vie, science des matériaux, synthèse chimique, chromatographie, analyse et dans de nombreux autres domaines..
Contacter notre Service technique
