This product is not soluble in either water or PBS. This product is tested for solubility at 1mg/mL in acetone. In addition, this product is also soluble in Chloroform at 10 mg/mL. A brief search of the literature reveals that a 1:1 mixture of DMSO and acidified isopropanol has been used successfully to dissolve this material.
M2003
MTT Formazan
powder
Synonyme(s) :
Thiazolyl blue formazan
Se connecter pour consulter les tarifs organisationnels et contractuels.
Sélectionner une taille de conditionnement
Changer de vue
| Taille/Référence | Disponibilité | Prix |
|---|---|---|
1 g | Veuillez contacter notre Service Clients pour connaître la disponibilité de ce produit. | 179,00 € |
A propos de cet article
Formule empirique (notation de Hill) :
C18H17N5S
Numéro CAS:
Poids moléculaire :
335.43
NACRES:
NA.47
PubChem Substance ID:
UNSPSC Code:
12171500
MDL number:
179,00 €
Veuillez contacter notre Service Clients pour connaître la disponibilité de ce produit.
Service technique
Besoin d'aide ? Notre équipe de scientifiques expérimentés est là pour vous.
Laissez-nous vous aiderassay
≥97% (TLC)
Quality Segment
form
powder
color
purple
solubility
acetone: 1 mg/mL
application(s)
diagnostic assay manufacturing
hematology
histology
storage temp.
room temp
SMILES string
Cc1nc(\N=N\C(=N\Nc2ccccc2)c3ccccc3)sc1C
InChI
1S/C18H17N5S/c1-13-14(2)24-18(19-13)23-22-17(15-9-5-3-6-10-15)21-20-16-11-7-4-8-12-16/h3-12,20H,1-2H3/b21-17+,23-22+
InChI key
GIFKVQOPAJDJLO-SZVLODPRSA-N
Biochem/physiol Actions
MTT assay is a colorimetric reaction, generally used to examine cell viability. It involves the enzymatic reduction of a yellow-colored, water-soluble, 3-[4,5-dimethylthiazole-2-yl]-2,5-diphenyltetrazolium bromide (MTT) to purple-colored, water-insoluble, MTT-formazan. This reaction is catalyzed by mitochondrial dehydrogenase and only occurs in viable cells. Thus, the amount of blue formazan is proportional to the number of viable cells. The absorbance of the formazan solution is analyzed spectrophotometrically at 570 nm.
1 of 1
Cet article | |||
|---|---|---|---|
| description powder | description MTT reduction end product | description MTT Cell Growth Assay is a colorimetric assay that can be used for either proliferation or complement-mediated cytotoxicity assays. | description MTT Cell Growth Assay is a colorimetric assay that can be used for either proliferation or complement-mediated cytotoxicity assays. |
| assay ≥97% (TLC) | assay - | assay - | assay - |
| solubility acetone: 1 mg/mL | solubility chloroform: 10 mg/mL, clear, dark violet | solubility - | solubility - |
| Quality Level 200 | Quality Level 100 | Quality Level 100 | Quality Level 100 |
| form powder | form powder | form - | form - |
| storage temp. room temp | storage temp. - | storage temp. 2-8°C | storage temp. 2-8°C |
| color purple | color - | color - | color - |
Still not finding the right product?
Explore all of our products under MTT Formazan
Classe de stockage
11 - Combustible Solids
Que se passe-t-il ?
WGK 3
Point d'éclair (°F)
Not applicable
Point d'éclair (°C)
Not applicable
EPI (équipement de protection individuelle)
Eyeshields, Gloves, type N95 (US)
Faites votre choix parmi les versions les plus récentes :
Déjà en possession de ce produit ?
Retrouvez la documentation relative aux produits que vous avez récemment achetés dans la Bibliothèque de documents.
-
What is the solubility of MTT Formazan, Product M2003, in water or PBS?
1 answer-
Helpful?
-
-
Is MTT formazan (M2003) used to measure cell viability? if it how to used it?
1 answer-
No. The MTT Formazan is the reduced or cleaved form of MTT and would not be suitable in determining cell viability. For viability or metabolic cell studies see product M5655.
https://www.sigmaaldrich.com/product/sigma/m5655Helpful?
-
-
What is the Department of Transportation shipping information for this product?
1 answer-
Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
Helpful?
-
-
Can MTT Formazan, Product M2003, be used in cell viability assays?
1 answer-
No. MTT or thiazolyl blue tetrazolium is used to determine the number of viable cells in well-cultures. Consider use of kit CGD1 or product M5655.
Helpful?
-
-
Can MTT Formazan, Product M2003, be used to measure dehydrogenases?
1 answer-
No. MTT is converted by active mitochondrial dehydrogenases of living cells to MTT Formazan. Consider use of product M2128 or M5655.
Helpful?
-
-
What is the proper wavelength for measuring MTT Formazan, Product M2003?
1 answer-
We recommend detection at 570 nm, with background subtraction at 630-690 nm in acidified isopropanol.
Helpful?
-