17490

Bromocresol Purple

for microscopy (Hist., Vit.), indicator (pH 5.2-6.8)

• Synonyme(s) : 5,5′-Dibromo-o-cresolsulfonphthalein, Bromcresol purple sultone form
• Formule empirique (notation de Hill): C₂₁H₁₆Br₂O₅S
• Numéro CAS: 115-40-2
• Poids moléculaire : 540.22
• Beilstein: 359618
• Numéro CE : 204-087-8
• Numéro MDL: MFCD00011681
• Code UNSPSC : 12171500
• ID de substance PubChem : 57647609
• Nomenclature NACRES : NA.47

Propriétés

• Qualité: for microscopy (Hist., Vit.); indicator (pH 5.2-6.8)
• Forme: powder
• Technique(s): titration: suitable
• pH: 5.2-6.8, yellow to purple
• Pf: 240 °C (dec.) (lit.)
• Solubilité: hot ethanol: 0.1 g/10 mL
• εmax: ≥400 at 414-424 nm in methanol
• Application(s): diagnostic assay manufacturing; hematology; histology
• Température de stockage: room temp
• Chaîne SMILES: Cc1cc(cc(Br)c1O)C2(OS(=O)(=O)c3ccccc23)c4cc(C)c(O)c(Br)c4
• InChI: 1S/C21H16Br2O5S/c1-11-7-13(9-16(22)19(11)24)21(14-8-12(2)20(25)17(23)10-14)15-5-3-4-6-18(15)29(26,27)28-21/h3-10,24-25H,1-2H3
• Clé InChI: ABIUHPWEYMSGSR-UHFFFAOYSA-N

Description

Description générale

Bromocresol purple is a pH indicator, which changes color from yellow (at low pH 5.2) to violet (above pH 6.8).[1] It aids in determining the dead cell count for cells with plasma membrane damage. Bromocresol purple is widely used as a fluorescent stain in yeast cells. Cells with an intact plasma membrane are not stained, while damaged cells are seen as blue-grey ghosts.[2]

Application

Spectrophotometric determination of serum-albumin

Informations sur la sécurité

• Code de la classe de stockage: 11 - Combustible Solids
• Classe de danger pour l'eau (WGK): WGK 3
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable
• Équipement de protection individuelle: dust mask type N95 (US), Eyeshields, Gloves