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Mélange phénol/chloroforme/alcool isoamylique

BioUltra, for molecular biology, 125:24:1

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About This Item

Numéro CAS:
136112-00-0
Numéro MDL:
MFCD00133763
Code UNSPSC :
12000000
ID de substance PubChem :
329767819
Nomenclature NACRES :
ND.02

Qualité

for molecular biology

Niveau de qualité

100

Gamme de produits

BioUltra

Forme

liquid

Impuretés

DNases, none detected
 RNases, none detected
 phosphatases, none detected
 proteases, none detected

pH

4-5(H2O-phase, after extraction with H2O, 1:1)

Densité

1.12 g/mL at 20 °C

λ

neat

Absorption UV

λ: 330 nm Amax: 0.2
λ: 405 nm Amax: 0.1
λ: 510 nm Amax: 0.1

Température de stockage

2-8°C

Chaîne SMILES 

ClC(Cl)Cl.CC(C)CCO.Oc1ccccc1

InChI

1S/C6H6O.C5H12O.CHCl3/c7-6-4-2-1-3-5-6;1-5(2)3-4-6;2-1(3)4/h1-5,7H;5-6H,3-4H2,1-2H3;1H

Clé InChI

ZYWFEOZQIUMEGL-UHFFFAOYSA-N

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Catégories apparentées

Application


  • Simultaneous quantification of multiple RNA cargos co-loaded into nanoparticle-based delivery systems: This research explores the application of the Phenol – chloroform – isoamyl alcohol mixture in extracting high-quality RNA for advanced therapeutic formulations, emphasizing its critical role in nanotechnology and pharmaceutical sciences (Lokras et al., 2022).

  • Analysis of Circulating microRNAs and Their Post-Transcriptional Modifications in Cancer Serum by On-Line Solid-Phase Extraction-Capillary Electrophoresis-Mass Spectrometry: Demonstrates the use of the Phenol – chloroform – isoamyl alcohol mixture for precise extraction of microRNAs from biological fluids, contributing to cancer diagnostics and molecular pathology (Pero-Gascon et al., 2018).

  • The application of alkaline lysis and pressure cycling technology in the differential extraction of DNA from sperm and epithelial cells recovered from cotton swabs: Highlights the integration of the Phenol – chloroform – isoamyl alcohol mixture with novel technologies for forensic applications, enhancing DNA extraction processes critical in legal and criminal investigations (Nori and McCord, 2015).

  • Frequency of Epstein-Barr virus DNA sequences in human gliomas: Utilizes the Phenol – chloroform – isoamyl alcohol mixture for DNA extraction in medical research, facilitating the study of viral presence in cancer tissues and its implications for oncology and virology (Fonseca et al., 2015).

  • The critical role of DNA extraction for detection of mycobacteria in tissues: This study underscores the effectiveness of the Phenol – chloroform – isoamyl alcohol mixture in the molecular detection of pathogens, serving as a fundamental technique in microbiological diagnostics and infectious disease research (Radomski et al., 2013).

Forme physique

mixture of phenol; chloroform; isoamyl alcohol in the ratio of 125:24:1 (v/v/v); saturated with 2 M NaOAc pH 4

Mention d'avertissement

Danger

Classification des risques

Acute Tox. 3 Dermal - Acute Tox. 3 Inhalation - Acute Tox. 3 Oral - Aquatic Chronic 2 - Carc. 2 - Eye Dam. 1 - Muta. 2 - Repr. 2 - Skin Corr. 1B - STOT RE 1 Oral - STOT RE 2

Organes cibles

Liver,Kidney, Nervous system,Kidney,Liver,Skin

Code de la classe de stockage

6.1A - Combustible acute toxic Cat. 1 and 2 / very toxic hazardous materials

Classe de danger pour l'eau (WGK)

WGK 3

Équipement de protection individuelle

Faceshields, Gloves, Goggles, type ABEK (EN14387) respirator filter

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Certificats d'analyse (COA)

Lot/Batch Number
BCCL1272
BCCJ9126
BCCD7662
BCBZ6964
BCBS4114V

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Wiesław Bogdanowicz et al.
Proceedings of the National Academy of Sciences of the United States of America, 106(30), 12279-12282 (2009-07-09)
We report the results of mitochondrial and nuclear DNA analyses of skeletal remains exhumed in 2005 at Frombork Cathedral in Poland, that are thought to be those of Nicolaus Copernicus (1473-1543). The analyzed bone remains were found close to the
Dae-Wook Yang et al.
Cell death and differentiation, 28(1), 233-250 (2020-08-02)
The microtubule network is crucial for cell structure and function. Patronin is a conserved protein involved in protecting the minus end of microtubules. Conversely, Klp10A is a kinesin-like microtubule depolymerase. Here we report the role of Drosophila Patronin and Klp10A

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