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05892970001

Roche

cOmplete ULTRA Tablets, Mini, EASYpack Protease Inhibitor Cocktail

Tablets supplied in foil blister packs.

Synonyme(s) :

protease inhibitor

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About This Item

Code UNSPSC :
12352204
Nomenclature NACRES :
NA.28

Forme

tablet

Conditionnement

pkg of 30 tablets (individually packed in foil blister packs)

Fabricant/nom de marque

Roche

Solubilité

water: soluble

Absorption

0.4 at 280 nm (1x solution)

Température de stockage

2-8°C

Description générale

cOmplete ULTRA Tablets contain the most powerful protease inhibitor cocktail for the optimal protection of your proteins against protease degradation. As this tablet contains EDTA, metalloproteases are also inhibited. They are supplied as tablets in an EASYpack blister pack. EASYpacks provide the most convenient form of delivery - single tablets are easily pushed through the blister foil directly into the buffer. One cOmplete ULTRA, Mini Tablet is sufficient for a volume of 10 ml extraction solution.
Recombinant proteins may be partially degraded during expression. Soluble degradation products are co-purified and cause a non-homogeneous protein preparation with protein fragments of different lengths. Metalloproteases, which are inhibited by EDTA, are fairly prevalent in, for example, bacteria.

You can easily inhibit both proteases and phosphatases at the same time by simply combining a cOmplete ULTRA, Mini, Protease Inhibitor Cocktail Tablet with a PhosSTOP Phosphatase Inhibitor Cocktail Tablet in the same 10 ml buffer.

Spécificité

Inhibits serine proteases, cysteine proteases. Does not inhibit metalloproteases completely when divalent cations like Ca2+, Mg2+, or Mn2+ are present

Application

During cell lysis and purification, target proteins are at risk of being cleaved by proteases, which are naturally present in cells. To ensure that full-length proteins are obtained, cOmplete ULTRA Tablets are used for the inhibition of an extended range of proteases. This new formulation shows highest inhibition ratios due to its broadened range of inhibitors compared to standard cOmplete Tablets.
This new protease inhibitor cocktail now inhibits aspartic proteases, as well as serine, cysteine, and metalloproteases. cOmplete ULTRA Tablets were developed for maximum efficiency on different cell types. Best performance is guaranteed in lysates of bacteria, yeast, insect cells, and a wide range of higher cell lines.
Roche ′s new resin for the purification of his-tagged proteins, cOmplete His-Tag Purification Resin, is compatible with this EDTA-containing tablet. Due to a new metal ion-binding-chelator chemistry in the cOmplete His-Tag Purification Resin, EDTA can be used during Immobilized Metal Ion Affinity Chromatography (IMAC).
cOmplete ULTRA Tablets, Mini, EASYpack Protease Inhibitor Cocktail has been used as a component of radioimmunoprecipitation assay buffer (RIPA buffer). It has also been used to prevent protein degradation and coagulation in blood samples.

Caractéristiques et avantages

  • Ultra efficient protection
The extended inhibitor cocktail composition makes this tablet an ideal formulation for your most difficult, protease-sensitive targets.
  • Convenient tablet format
Eliminate weighing small amounts, and instantly protect your proteins against an exceptionally wide range of proteases by simply pushing a tablet through the EASYpack foil packaging directly into your buffers.
  • Suitable for IMAC purification
Purify poly-histidine tagged proteins.
  • Ensure laboratory safety
Avoid contact with hazardous compounds using cOmplete ULTRA Tablets.

Composants

Proprietary mixture of several protease inhibitors with broad inhibitory specificity.

Forme physique

Tablets in foil blister packs. Each tablet is sufficient for a volume of 10 ml extraction solution.

Notes préparatoires

Working concentration: 1 tablet per 10 ml extraction solution.
Working solution: Handling

Carefully push the tablet through the foil packaging using the base of your thumb (not your fingernail) to prevent the breakage of tablets.

Preparation of Working Solution

One tablet is sufficient for the inhibition of proteolytic activity in 10 ml extraction solution. If very high proteolytic activity is present, 2-3 tablets should be used for 10 ml extraction buffer. The tablets can be added directly to the extraction medium.

Stock Solution (2x conc.)

Alternatively to the direct use with lysates, a 2x stock solution can be prepared, with the following procedure:
• Add cOmplete ULTRA Tablet to 5 ml double-distilled water in a PE or PP tube.
• Vortex 3 min or until the tablet is completely dissolved.
• The stock solution is ready to use even if there are very fine parti­cles still visible in solution. These particles do not interfere with the inhibition performance of the tablets.
• Stock solution is stable for 4 weeks, stored at 2 to 8 °C, and for at least 4 weeks at  -15 to  -25 °C as well.
• During storage of the 2x stock solution, the particles can sediment. Briefly vortex the stock solution prior to use.

Storage conditions (working solution): The stock solution in water is stable for 4 weeks, stored at 2 to 8 °C, and for at least 4 weeks at  -15 to -25 °C as well.

Reconstitution

Soluble in aqueous buffers, or add directly to extraction media.

For the stock solution, use water only.

Autres remarques

For life science research only. Not for use in diagnostic procedures.

Informations légales

cOmplete is a trademark of Roche

Pictogrammes

Corrosion

Mention d'avertissement

Danger

Mentions de danger

Classification des risques

Aquatic Chronic 3 - Eye Dam. 1 - Skin Corr. 1B

Code de la classe de stockage

8A - Combustible, corrosive hazardous materials

Classe de danger pour l'eau (WGK)

WGK 2

Point d'éclair (°F)

does not flash

Point d'éclair (°C)

does not flash


Certificats d'analyse (COA)

Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".

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Consulter la Bibliothèque de documents

Yukiko Yasuoka et al.
Physiological reports, 8(12), e14485-e14485 (2020-06-28)
The detection of erythropoietin (Epo) protein by Western blotting has required pre-purification of the sample. We developed a new Western blot method to detect plasma and urinary Epo using deglycosylation. Epo in urine and tissue, and erythropoiesis-stimulating agents (ESAs) in
Pharmacokinetic parameters and mechanism of action of an efficient anti-A$\beta$ single chain antibody fragment
Esquerda-Canals G, et al.
Testing, 14(5), e0217793-e0217793 (2019)
Yinan Li et al.
eLife, 9 (2020-10-02)
Terminal selectors are transcription factors (TFs) that establish during development and maintain throughout life post-mitotic neuronal identity. We previously showed that UNC-3/Ebf, the terminal selector of C. elegans cholinergic motor neurons (MNs), acts indirectly to prevent alternative neuronal identities (Feng
Jesse J Balic et al.
Nature communications, 11(1), 3816-3816 (2020-08-01)
Detection of microbial components such as lipopolysaccharide (LPS) by Toll-like receptor 4 (TLR4) on macrophages induces a robust pro-inflammatory response that is dependent on metabolic reprogramming. These innate metabolic changes have been compared to aerobic glycolysis in tumour cells. However
Ngang Heok Tang et al.
Current biology : CB, 30(5), 865-876 (2020-01-28)
mRNA decay factors regulate mRNA turnover by recruiting non-translating mRNAs and targeting them for translational repression and mRNA degradation. How mRNA decay pathways regulate cellular function in vivo with specificity is poorly understood. Here, we show that C. elegans mRNA decay factors

Contenu apparenté

Select different protease inhibitor types based on your needs to prevent protein degradation during isolation and characterization and safeguard proteins in sample prep.

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