PK1010

PhosphoDetect Anti-Syk (pTyr^525/526) Rabbit pAb

liquid, Calbiochem^®

• Code UNSPSC : 12352203
• Nomenclature NACRES : NA.41

Propriétés

• Source biologique: rabbit
• Niveau de qualité: 100
• Forme d'anticorps: affinity isolated antibody
• Type de produit anticorps: primary antibodies
• Clone: polyclonal
• Forme: liquid
• Ne contient pas: preservative
• Espèces réactives: human, mouse
• Fabricant/nom de marque: Calbiochem^®
• Conditions de stockage: OK to freeze
• Isotype: IgG
• Conditions d'expédition: wet ice
• Température de stockage: −20°C
• Modification post-traductionnelle de la cible: phosphorylation (pTyr525/pTyr526)
• Informations sur le gène: human ... SYK(6850)

Description

Description générale

Protein A and immunoaffinity purified rabbit polyclonal antibody. Recognizes the ~72 kDa Syk protein phosphorylated on Tyr^525/526.

Recognizes the ~72 kDa Syk protein phosphorylated at Tyr^525/526 in human Syk or Tyr^519/520 in mouse Syk in 293T cells expressing wild-type Syk and CD8.

This PhosphoDetect Anti-Syk (pTyr^525/526) Rabbit pAb is validated for use in ELISA, Immunoblotting for the detection of Syk (pTyr^525/526).

Immunogène

Human

a synthetic phosphopeptide corresponding to amino acids surrounding the Tyr^525/526 phosphorylation sites of human Syk

Application

ELISA (1:1000)
Immunoblotting (1:1000)

Avertissement

Toxicity: Standard Handling (A)

Forme physique

In 150 mM NaCl, 10 mM sodium HEPES, 100 µg/ml BSA, 50% glycerol, pH 7.5.

Reconstitution

Do not aliquot.

Remarque sur l'analyse

Positive Control
293T cells transfected with wild-type Syk and CD8

Autres remarques

Coopman, P.J., et al. 2000. Nature406, 742.
Turner, M., et al. 2000. Immunol. Today21, 148.
Zang, J., et al. 2000. J. Biol. Chem.275, 35442.
Chu, D.H., et. al. 1998. Immunol. Rev.165, 167.
Cheng, A.M. and Chan, A.C. 1997. Curr. Opin. Immunol.9, 528.
Kurosaki, T. 1997. Curr. Opin. Immunol.9, 309.

Tyr^525/526 represent the phosphorylation sites in human Syk, while Tyr^519/520 are the equivalent phosphorylation sites in mouse Syk. For immunoblotting the membrane should be incubated with antibody diluted in 5% BSA, 1X TBS, 0.1% Tween^®-20 detergent at 4°C overnight with gentle shaking. Antibody should be titrated for optimal results in individual systems.

Informations légales

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

TWEEN is a registered trademark of Croda International PLC

Informations sur la sécurité

• Code de la classe de stockage: 10 - Combustible liquids
• Classe de danger pour l'eau (WGK): WGK 2