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71754-M

Sigma-Aldrich

Terrific Broth - Novagen

Terrific Broth is a highly enriched granulated medium to improve the yield of plasmid DNA from E. coli.

Synonyme(s) :

TB media

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About This Item

Code UNSPSC :
41106213
Nomenclature NACRES :
NA.54
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Niveau de qualité

200

Forme

granular

Fabricant/nom de marque

Novagen®

Conditions de stockage

OK to freeze

Technique(s)

microbiological culture: suitable

Application(s)

microbiology

Température de stockage

10-30°C

Description générale

Terrific Broth (TB) is one of the complex media that is widely used to grow Escherichia coli in small-scale cultures. [1] Terrific Broth is a highly enriched granulated medium to improve the yield of plasmid DNA from E. coli. The granules ensure rapid and uniform dissolution in water, and prevent clumping of the medium and inhalation of the airborne powder. The composition of the Terrific Broth per liter is 12 g tryptone, 24 g yeast extract, 9.4 g potassium phosphate, dibasic, and 2.2 g potassium phosphate, monobasic. Use 47.6 g of granulated medium per liter of water.

Application

Terrific Broth – Novagen has been used to culture BLR(DE3) bacteria for protein polymer synthesis[2] and production of recombinant antigens for autoantibody assays[3]

Caractéristiques et avantages

  • Media preparation is simple and quick
  • ready to use EasyPak media packets: Just add water and sterilize
  • Consistent, high-quality media for E.coli cells

Avertissement

Toxicity: Standard Handling (A)

Informations légales

NOVAGEN is a registered trademark of Merck KGaA, Darmstadt, Germany

Code de la classe de stockage

11 - Combustible Solids

Classe de danger pour l'eau (WGK)

WGK 3

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A Murray et al.
Annals of oncology : official journal of the European Society for Medical Oncology, 21(8), 1687-1693 (2010-02-04)
Publications on autoantibodies to tumour-associated antigens (TAAs) have failed to show either calibration or reproducibility data. The validation of a panel of six TAAs to which autoantibodies have been described is reported here. Three separate groups of patients with newly
Liese N Beenken-Rothkopf et al.
Journal of biomaterials applications, 28(3), 395-406 (2012-07-27)
Protein polymer-based hydrogels have shown potential for tissue engineering applications, but require biocompatibility testing for in vivo use. Enzymatically crosslinked protein polymer-based hydrogels were tested in vitro and in vivo to evaluate their biocompatibility. Endotoxins present in the hydrogel were removed by Trition
Mario Losen et al.
Biotechnology progress, 20(4), 1062-1068 (2004-08-07)
Shake-flask cultures are widely used for screening of high producing strains. To select suitable strains for production scale, cultivation parameters should be applied that provide optimal growth conditions. A novel method of measuring respiratory activity in shake-flask cultures was employed

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