L1900

Luria broth base, Miller′s modified

Tryptone, yeast extract and sodium chloride

• Synonyme(s) : Lennox broth base, modified, Luria-Bertani broth base, modified, Lysogeny broth base, modified
• Code UNSPSC : 41106200
• Nomenclature NACRES : NA.72

Propriétés

• Forme: powder
• Technique(s): microbiological culture: suitable
• Application(s): agriculture; microbiology

Description

Description générale

Luria broth (LB) is a nutritionally rich bacterial culture medium that is commonly used for maintaining and cultivating laboratory recombinant strains of E. coli. It has potential applications in molecular microbiology methods such as the preparation of plasmid DNA and recombinant proteins. Luria broth base, Miller′s modified is used to culture members of the family Enterobacteriaceae and for coliphage plaque assays.

Application

Luria broth base, Miller′s modified has been used as a nutrient culture medium for E-coli cells.

Luria broth base (LB), Miller (L1900) is a bacteria growth mixture of sodium chloride (0.5g/L); tryptone (10g/L) and yeast extract (5g/L) typically combined with agar (15g/L).

Composants

Contains: Tryptone, yeast extract and sodium chloride.

Reconstitution

Recommended use at 15.5 g per liter.

Informations sur la sécurité

• Code de la classe de stockage: 10 - Combustible liquids
• Classe de danger pour l'eau (WGK): WGK 3
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable