204882

Complement C2, Human

Complement C2, Human, is a native, single chain glycoprotein present. C2 is cleaved by the activated C1s into C2a, the 63 kDa C-terminal fragment, and C2b, the 30 kDa N-terminal fragment.

• Synonyme(s) : C2 Protein
• Code UNSPSC : 12352202
• Nomenclature NACRES : NA.25

Propriétés

• Niveau de qualité: 100
• Pureté: ≥90% (SDS-PAGE)
• Forme: liquid
• Activité spécifique: ≥4×10⁵ C2H50 units/mg (>=70% of the C2 activity in normal human serum on a mg/mg basis)
• Fabricant/nom de marque: Calbiochem^®
• Conditions de stockage: OK to freeze; avoid repeated freeze/thaw cycles
• Impuretés: C1, C3, C4, C5, IgG, IgA, IgM, albumin, Factor B, or hemopexin, trace
• Conditions d'expédition: wet ice
• Température de stockage: −70°C

Description

Description générale

Native, human C2 complement component. Single chain glycoprotein present in normal human serum at concentration of 20 µg/ml. Classical pathway activation results in assembly of the C1,C4b complex on target surfaces. Subsequent to binding of the C4b component to this complex, C2 is cleaved by the activated C1s subcomponent of C1 into C2a, the 63 kDa C-terminal fragment, and C2b, the 30 kDa N-terminal fragment. The smaller C2b fragment is released while the larger C2a fragment remains associated with the C1,C4b complex to form the C4b,C2a classical pathway C3 convertase enzyme. C2 concentration is based on functional activity measurements relative to a normal human serum standard pool.

Conditionnement

Please refer to vial label for lot-specific concentration.

Avertissement

Toxicity: Standard Handling (A)

Forme physique

In PBS, pH 6.0.

Notes préparatoires

Prepared from plasma that has been shown by certified tests to be negative for HBsAg and for antibodies to HIV and HCV.

Reconstitution

Following initial thaw, aliquot and freeze (-70°C).

Autres remarques

Attwood, J.T., et al. 2001. Acta Paediatr.90, 99.

Informations légales

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Informations sur la sécurité

• Code de la classe de stockage: 12 - Non Combustible Liquids
• Classe de danger pour l'eau (WGK): nwg
• Point d'éclair (°F): Not applicable
• Point d'éclair (°C): Not applicable