1.25079

Fractogel^® EMD DEAE (M)

MiniChrom Fractogel^® DEAE (M), volume 5 mL, prepacked column

• Synonyme(s) : MiniChrom Column Fractogel^® DEAE (M), 5 ml
• Code UNSPSC : 41115711

Propriétés

• ligand: diethyl-(2-hydroxypropyl)aminoethyl
• Niveau de qualité: 100
• Matériaux: polypropylene housing
• Gamme de produits: Fractogel^®
• Forme: prepacked column
• Paramètres: ≤8 bar pressure; ~170 cm/hr max. flow rate
• Longueur du lit: 100 mm
• Volume du lit: 5 mL
• D.I. de la colonne: 8 mm
• Volume: 5 mL
• Groupe de la matrice active: Methacrylate
• Taille moyenne des particules: 40-90 μm
• Capacité: ~100 mg, BSA binding capacity
• Application(s): gene therapy; mAb; vaccine development
• Technique de séparation: anion exchange
• Température de stockage: 15-25°C

Description

Description générale

Weak cation exchange Fractogel^® EMD DEAE (M) resin in a convenient pre-packed 5 ml column

Caractéristiques et avantages

MiniChrom Column Fractogel^® DEAE (M), 5 ml enables:

• Excellent binding to large viruses and plasmid DNA
• Homogenous binding with high selectivity and purity
• Lower elution volumes for the highest purity levels
• Compatibility with 2.5 % (v/v) aqueous benzyl alcohol containing 150 mM NaCl storage solution

Due to the titration behavior, the ion exchange capacity can be used from pH 2 to pH 9.5. The separation of proteins is based on reversible electrostatic interactions between the negatively charged regions of the proteins′ surface and the support. Proteins are retained efficiently on Fractogel^® EMD DEAE when the pH of the buffer is about 1 unit above their isoelectric points (pl).

The strength of the binding depends on the following:

• the buffer system
• pH value of the buffer which determines the surface charge of the protein
• the degree of the ionization of the functional groups of the exchanger
• the concentration of the counter ions
• the charge density on the support (protein binding capacity)

Informations légales

FRACTOGEL is a registered trademark of Merck KGaA, Darmstadt, Germany