861030

Auramine O

Dye content 85 %, certified by the BSC

• Synonyme(s) : 4,4′-(Imidocarbonyl)bis(N,N-dimethylaniline) monohydrochloride, Basic Yellow 2, Pyoctaninum aureum
• Formule empirique (notation de Hill) : C₁₇H₂₁N₃ · HCl
• Numéro CAS: 2465-27-2
• Poids moléculaire : 303.83
• Numéro C.I. (Colour Index): 41000
• Beilstein: 4030061
• Numéro CE : 219-567-2
• Numéro MDL: MFCD00012484
• Code UNSPSC : 12171500
• ID de substance PubChem : 24888527
• Nomenclature NACRES : NA.47

Propriétés

• Agence: certified by the BSC
• Niveau de qualité: 200
• Forme: powder or chunks
• Composition: Dye content, 85%
• Technique(s): microbe id | staining: suitable
• Pf: >250 °C (dec.) (lit.)
• Solubilité: 95% ethanol: 1 mg/mL, clear to hazy, yellow to very deep yellow
• λmax: 370 nm; 434 nm (2nd)
• ε (coefficient d'extinction): ≥12000 at 249-255 nm in water at 0.005 g/L; ≥15000 at 367-373 nm in water at 0.005 g/L; ≥36000 at 429-435 nm in water at 0.005 g/L
• Application(s): diagnostic assay manufacturing; hematology; histology
• Température de stockage: room temp
• Chaîne SMILES: Cl[H].CN(C)c1ccc(cc1)C(=N)c2ccc(cc2)N(C)C
• InChI: 1S/C17H21N3.ClH/c1-19(2)15-9-5-13(6-10-15)17(18)14-7-11-16(12-8-14)20(3)4;/h5-12,18H,1-4H3;1H
• Clé InChI: KSCQDDRPFHTIRL-UHFFFAOYSA-N

Description

Application

Auramine O has been used to study the cuticle/cutin in the epidermis of the pollen presenter in Vangueria infausta.[1]

Actions biochimiques/physiologiques

Auramine O is used for the staining of acid-fast organisms, coccidia.[2] Auramine O along with carbol generates brilliant yellow fluorochrome of tubercle bacilli. It is effective in detecting positive cases of tuberculosis.[3] Auramine O binds to the mycolic acid in the bacterial cell wall.[4]

Adéquation

Certified for use by fluorescence microscopy in Churukian′s modification of Truant′s fluorescent method for acid fast bacilli on paraffin sections.

Informations sur la sécurité

• Pictogrammes: GHS08,GHS07,GHS09
• Mention d'avertissement: Warning
• Mentions de danger: H302,H319,H351,H411
• Conseils de prudence: P201 - P273 - P301 + P312 + P330 - P305 + P351 + P338 - P308 + P313
• Classification des risques: Acute Tox. 4 Oral - Aquatic Chronic 2 - Carc. 2 - Eye Irrit. 2
• Code de la classe de stockage: 6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects
• Classe de danger pour l'eau (WGK): WGK 3