ABS2249

Anti-SNORC

purified antibody, from rabbit

• NACRES: NA.41
• UNSPSC Code: 12352203
• Clone: polyclonal
• Species reactivity: mouse
• Application: ICC, IHC (p), WB
• Citations: -

Properties

• biological source: rabbit
• Quality Level: 200
• antibody form: purified antibody
• antibody product type: primary antibodies
• clone: polyclonal
• mol wt: calculated mol wt 12.18 kDa, observed mol wt ~22 kDa
• species reactivity: mouse
• packaging: antibody small pack of 100 μg
• technique(s): immunocytochemistry: suitable, immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable, western blot: suitable
• isotype: IgG
• shipped in: dry ice
• storage temp.: 2-8°C
• target post-translational modification: unmodified

Description

General description

Protein SNORC (UniProt: Q9CXL7; also known as Secondary ossification center-associated regulator of chondrocyte maturation protein) is encoded by the Snorc gene (Gene ID: 73234) in murine species. SNORC is a highly conserved, single-pass membrane protein that is synthesized with a signal peptide (aa 1-24), which is subsequently cleaved off to generate the mature form that contains an extracellular domain (aa 25-92), a transmembrane domain (aa 93-113), and a short cytoplasmic domain (aa 114-121). It contains a highly conserved glycosaminoglycan (GAG) attached to serine 44. Its expression is observed only in cartilage and plays a role in the regulation of chondrocyte maturation and postnatal endochondral ossification. It is reported that SNORC is not essential during early differentiation and its function pertains more to mature chondrocytes. (Ref.: Jaiswal, PK., et al. (2020). Sci. Rep. 10; Article 7790).

Immunogen

His-tagged recombinant fragment corresponding to 67 amino acids from the extracellular domain of murine SNORC protein.

Application

Quality Control TestingEvaluated by Western Blotting in lysate from HEK293 cells expressing the untagged Mouse SNORC.Western Blotting Analysis: A 1:1,000 dilution of this antibody detected SNORC in lysate from HEK293 cells expressing the untagged Mouse SNORC.Tested ApplicationWestern Blotting Analysis: A 1:10,000 dilution from a representative lot detected SNORC in 3xFlag mouse-SNORC (Courtesy of Dr. Pierre Moffatt; Shriners Hospitals for Children and McGill University, Montreal, Canada).Immunocytochemistry Analysis: A representative lot detected SNORC in Immunocytochemistry applications (Jaiswal, P.K., et. al. (2020). Sci Rep. 10(1):7790).Immunohistochemistry (Paraffin) Analysis: A 1:3,000 dilution from a representative lot detected SNORC in Mouse Decalcified Femur (Global KO) (Courtesy of Dr. Pierre Moffatt; Shriners Hospitals for Children and McGill University, Montreal, Canada).Immunohistochemistry Applications: A representative lot detected SNORC in Immunohistochemistry applications (Jaiswal, P.K., et. al. (2020). Sci Rep. 10(1):7790).Western Blotting Analysis: A representative lot detected SNORC in Western Blotting applications (Jaiswal, P.K., et. al. (2020). Sci Rep. 10(1):7790).Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user

Anti-SNORC, Cat. No. ABS2249, is a rabbit polyclonal antibody that detects SNORC and is tested for use in Western Blotting, Immunocytochemistry, Immunohistochemistry (Paraffin), and Western Blotting.

Biochem/physiol Actions

This rabbit polyclonal antibody detects secondary ossification center-associated regulator of chondrocyte maturation protein (SNORC). It targets an epitope within 67 amino acids from the extracellular domain.

Physical form

Purified rabbit polyclonal antibody in PBS with 0.05% sodium azide.

Preparation Note

Stable for 1 year at +2°C to +8°C from date of receipt.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class: 12 - Non Combustible Liquids
• wgk: WGK 2
• flash_point_f: Not applicable
• flash_point_c: Not applicable