06-342

Anti-JAK3 Antibody

serum, Upstate^®

• UNSPSC Code: 12352203
• NACRES: NA.41
• eCl@ss: 32160702
• Conjugate: unconjugated
• Clone: polyclonal
• Application: WB
• Citations: 36

Properties

• biological source: rabbit
• Quality Level: 100
• conjugate: unconjugated
• antibody form: serum
• antibody product type: primary antibodies
• clone: polyclonal
• species reactivity: mouse
• manufacturer/tradename: Upstate^®
• technique(s): western blot: suitable
• isotype: IgG
• NCBI accession no.: NM_000215
• UniProt accession no.: P52333
• shipped in: wet ice
• target post-translational modification: unmodified
• Gene Information: mouse ... Jak3(16453)

Description

General description

125 kDa

Immunogen

peptide corresponding to residues 934-952 (RDELCVAGAQLYACQDPAI) of murine JAK3. The immunizing peptide sequence shares 16/19 amino acids with rat JAK3, but contains an amino acid insertion, which may block cross-reactivity. The immunizing peptide shares 12/19 amino acids with human JAK3.

Application

Anti-JAK3 Antibody is an antibody against JAK3 for use in WB.

Biochem/physiol Actions

: Recognizes murine JAK3, Mr 125kDa and JAK3 degradation products, Mr 95-120kDa. The antiserum shows very poor immunoreactivity for human JAK3. No immunoreaction was noted with immuno-precipitated mouse JAK1 and JAK2

This antibody has been tested on several human cell lines including A431, Hela, Jurkat, and HEK293 cells. The antiserum shows very poor immunoreactivity for human JAK3

Physical form

antiserum containing 0.05% sodium azide

Preparation Note

2 years at -20°C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery, centrifuge the original vial after thawing and prior to removing the cap.

Analysis Note

routinely evaluated by immunoblot on lysate from a mouse cytotoxic T lymphocyte cell line (CTLL)

Legal Information

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

Safety Information

• Storage Class: 10 - Combustible liquids
• wgk: WGK 1