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HPLC Analysis of Sugars on Ascentis® Si

HPLC Analysis of Sugars on Ascentis® Si application for HPLC

Materialien

Analytische Säule

Produkt-Nr.
Beschreibung
Preisangaben

581512-U

Ascentis® Si HPLC-Säule

5 μm particle size, L × I.D. 15 cm × 4.6 mm

Standard

Produkt-Nr.
Beschreibung
Preisangaben

M8378

Maltotriose

≥90% (HPLC)

84097

Saccharose

BioUltra, for molecular biology, ≥99.5% (HPLC)

47287-U

Lactosemonohydrat

analytical standard

G0350500

Glucose

European Pharmacopoeia (EP) Reference Standard

X0200000

Xylose

European Pharmacopoeia (EP) Reference Standard

1375025

Maltose Monohydrat

United States Pharmacopeia (USP) Reference Standard

CONDITIONS

column

Ascentis Si, 15 cm x 4.6 mm I.D., 5 μm particles (581512-U)

mobile phase

[A] water; [B] acetonitrile; (15:85, A:B)

flow rate

1.0 mL/min

column temp.

35 °C

detector

ELSD, 55 °C, 3.5 bar nitrogen

injection

10 μL

Beschreibung

Hinweis zur Analyse

This application demonstrates the suitability of the Ascentis Si for the analysis of sugars. Structures along with the optimized chromatogram obtained on the Ascentis Si are presented. Separation is by normal-phase mechanism as a decrease in mobile phase water content results in increased retention.

Rechtliche Hinweise

Ascentis is a registered trademark of Merck KGaA, Darmstadt, Germany

Chromatogram Search

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