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Mechanical regulation of cardiac fibroblast profibrotic phenotypes.

Molecular biology of the cell (2017-05-05)
Kate M Herum, Jonas Choppe, Aditya Kumar, Adam J Engler, Andrew D McCulloch
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Cardiac fibrosis is a serious condition currently lacking effective treatments. It occurs as a result of cardiac fibroblast (CFB) activation and differentiation into myofibroblasts, characterized by proliferation, extracellular matrix (ECM) production and stiffening, and contraction due to the expression of smooth muscle α-actin. The mechanical properties of myocardium change regionally and over time after myocardial infarction (MI). Although mechanical cues are known to activate CFBs, it is unclear which specific mechanical stimuli regulate which specific phenotypic trait; thus we investigated these relationships using three in vitro models of CFB mechanical activation and found that 1) paracrine signaling from stretched cardiomyocytes induces CFB proliferation under mechanical conditions similar to those of the infarct border region; 2) direct stretch of CFBs mimicking the mechanical environment of the infarct region induces a synthetic phenotype with elevated ECM production; and 3) progressive matrix stiffening, modeling the mechanical effects of infarct scar maturation, causes smooth muscle α-actin fiber formation, up-regulation of collagen I, and down-regulation of collagen III. These findings suggest that myocyte stretch, fibroblast stretch, and matrix stiffening following MI may separately regulate different profibrotic traits of activated CFBs.

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Monoklonaler Anti-Vinculin-Antikörper in Maus hergestellte Antikörper, clone hVIN-1, ascites fluid
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Anti--α-Glattmuskel-Aktin-Antikörper, Maus, monoklonal, clone 1A4, purified from hybridoma cell culture
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N-(3-Dimethylaminopropyl)-N′-ethyl-carbodiimid, ≥97.0% (T)
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Ziegenserum aus kontrollierter Spenderherde, USA origin, sterile-filtered, suitable for cell culture
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GW2580, ≥98% (HPLC)