Direkt zum Inhalt
Merck

SMAD3 Regulates Follicle-stimulating Hormone Synthesis by Pituitary Gonadotrope Cells

The Journal of biological chemistry (2016-12-21)
Yining Li, Gauthier Schang, Ulrich Boehm, Chu-Xia Deng, Jonathan Graff, Daniel J Bernard
ZUSAMMENFASSUNG

Pituitary follicle-stimulating hormone (FSH) is an essential regulator of fertility in females and of quantitatively normal spermatogenesis in males. Pituitary-derived activins are thought to act as major stimulators of FSH synthesis by gonadotrope cells.

MATERIALIEN
Produktnummer
Marke
Produktbeschreibung

Sigma-Aldrich
Hyaluronidase aus Rindertestes, Type IV-S, lyophilized powder (essentially salt-free), 750-3000 units/mg solid
Sigma-Aldrich
2,2,2-Tribromethanol, 97%
Sigma-Aldrich
Choriongonadotropin, lyophilized powder, vial of ~2,500 IU
Sigma-Aldrich
Paraformaldehyd, reagent grade, crystalline
Sigma-Aldrich
Albumin aus Rinderserum, lyophilized powder, 1× crystallized, ≥97% (agarose gel electrophoresis)
Sigma-Aldrich
Medium 199, With Hanks′ salts and sodium bicarbonate, without L-glutamine, liquid, sterile-filtered, suitable for cell culture