C1063

Choriongonadotropin

lyophilized powder, vial of ~2,500 IU

• Synonym(e): Choriogonin, HCG
• CAS-Nummer: 9002-61-3
• UNSPSC Code: 12352209
• NACRES: NA.32
• MDL number: MFCD00130797
• Biological source: human urine
• Form: lyophilized powder

Eigenschaften

• biological source: human urine
• Quality Segment: 200
• sterility: sterile-filtered
• form: lyophilized powder
• mol wt: 37.9 kDa
• contains: mannitol
• packaging: vial of ~2,500 IU
• drug control: Home Office Schedule 4.2
• impurities: HBsAg, HCV and HIV-1/HIV-2, tested negative
• solubility: water: 10 mL/vial, clear, colorless
• UniProt accession no.: P01215
• shipped in: ambient
• storage temp.: −20°C
• Gene Information: human ... CGA(1081)

Beschreibung

General description

Das Glykoproteinhormon, das aus einer α-Kette mit 92 Aminosäuren besteht, die der von LH, FSH und TSH entspricht; und einer eindeutigen β-Kette mit 149 Aminosäuren. Wird produziert vom Chorionzottengewebe und ist verantwortlich für die Aufrechterhaltung des Gelbkörpers während der frühen Schwangerschaft. Wird auch von Throphoblastzellenneoplasmen produziert, die sich durch erhabene, freie hCG-β-Ketten und hyperglykosyliertem hCG auszeichnen.

Application

Chorionic gonadotropin human has been used in the biological validation of urinary testosterone (UTM) and urinary estradiol metabolites (UEM) EIA and superovulation.

Biochem/physiol Actions

Human chorionic gondadotropin (hCG) may be used as an alternative to PMSG to induce ovulation in laboratory animals.[1] When hCG was injected into laboratory animals via intravenous (i.v.), intraperitoneal (i.p.), or subcutaneous (s.c.) methodstet, the effective dose values for 50% of the animals (ED50) per animal and per body weight (kg) in parenthesis were as follows:
Mice: i.v. 0.2 (7.7) I.U.; i.p. 0.3 (11.5) I.U.; s.c. 0.7 (26.9) I.U.
Syrian hamsters: i.v. 1.0 (9.5) I.U.; i.p. 1.8 (17.1) I.U.; s.c. 2.6 (24.8) I.U.
Rats: i.v. 1.3 (4.6) I.U.; i.p. 3.5 (12.3) I.U.; s.c.7.5 (26.3) I.U.

Using PMSG, the ED50 values per animal and per body weight (kg) in parenthesis were as follows:
Mice: i.v. 0.8 (30.8) I.U.; i.p. 2.0 (76.9) I.U.; s.c. 2.8 (107.7) I.U
Syrian hamsters: i.v., 3.6 (34.3) I.U.; i. p., 8.0 (76.2) I.U.; s.c., 13.2 (125.7) I.U.
Rats: i.v., 6.0 (76.8) I.U.; i p., 20.8 (73.0) I.U.; s.c., 76.8 (269.5) I.U.

Preparation Note

When reconstituted with water to a concentration of 1,000 IU/mL, the vial will also contain 0.04 M sodium phosphate buffer (pH approx. 7.2) and 40 mg/mL of mannitol.

Analysis Note

The calculated value is based on the USP method for human Chorionic Gonadotrophin, to meet 2,500 IU per vial, with a tolerance of not less than 80.0% and not more than 125.0% of the potency stated.

Sicherheitshinweise

• Lagerklasse: 11 - Combustible Solids
• wgk: WGK 2
• flash_point_f: Not applicable
• flash_point_c: Not applicable