Lung Section Staining and Microscopy.

Bio-protocol (2017-11-25)

Xiaofeng Zhou, Bethany B Moore

ZUSAMMENFASSUNG

Our protocol describes immunofluorescent staining, hematoxylin and eosin staining and Masson's trichrome staining on lung sections.

MATERIALIEN

Produktnummer

Marke

Produktbeschreibung

Sigma-Aldrich

Essigsäure, glacial, ReagentPlus^®, ≥99%

Sigma-Aldrich

Salzsäure, ACS reagent, 37%

Sigma-Aldrich

Eisen(III)-chlorid, reagent grade, 97%

Sigma-Aldrich

Zitronensäure, ACS reagent, ≥99.5%

Sigma-Aldrich

Aluminium-Kaliumsulfat Dodecahydrat, ACS reagent, ≥98%

Sigma-Aldrich

Hämatoxylin, certified by the Biological Stain Commission

Sigma-Aldrich

Eosin Y, Dye content ~99 %

Sigma-Aldrich

Natriumphosphat, BioXtra, ≥99.0%