SRP4651

PTHrP human

recombinant, expressed in E. coli, ≥98% (SDS-PAGE), ≥98% (HPLC)

• Synonym(e): HHM, PLP, PTHLH, PTHR, PTHRP
• UNSPSC Code: 51111800
• NACRES: NA.32
• MDL number: MFCD01635684

Eigenschaften

• biological source: human
• recombinant: expressed in E. coli
• assay: ≥98% (HPLC), ≥98% (SDS-PAGE)
• form: lyophilized
• mol wt: ~9.8 kDa
• packaging: pkg of 50 μg
• storage condition: avoid repeated freeze/thaw cycles
• impurities: endotoxin, tested
• NCBI accession no.: P12272
• shipped in: wet ice
• storage temp.: −20°C
• Gene Information: human ... PTHLH(5744)

Beschreibung

General description

Parathyroid Hormone-like related Protein (PTHrP) is a member of the Parathyroid hormone family that is expressed in the mammary gland. It is known as a potent inhibitor of chondrocyte maturation. PTHrP is expressed by periarticular perichondrial cells and exerts its biological activity on PP-R expressing cells. During endochondral bone development PTHrP plays a major role in suppressing the onset of hypertrophic differentiation. It is also responsible for most cases of humoral hypercalcemia of malignancy. Recombinant Human PTHrP is a 9.8 kDa protein consisting of 86 amino acid residues.

Recombinant Human PTHrP is a 9.8 kDa protein consisting of 86 amino acid residues.

Application

The human parathyroid hormone-like related protein (PTHrP) has been used as an osteoarthritis-associated factor regulating hypertrophic differentiation of human chondrocytes. It has also been used in the regulation of the expression of Smpd3 (Sphingomyelin phosphodiesterase 3) in chondrocytes.

Biochem/physiol Actions

The gene PTHrP (parathyroid hormone-like related protein) encodes a protein involved in skeletal development. It binds to the receptor activated by PTH (parathyroid hormone) and functions in smooth muscle relaxation, regulation of cellular proliferation and differentiation. It also regulates endochondral bone development and proliferation of growth plate chondrocytes. It regulates fetal blood calcium and placental calcium transport. It has been associated with malignant hypercalcemia. Mutation in this gene has been associated with brachydactyly type E2 (BDE2).

Physical form

Sterile filtered and lyophilized without additives.

Preparation Note

Centrifuge the vial prior to opening. Avoid freeze-thaw cycles.

Reconstitute in water to a concentration of 0.1-1.0 mg/mL. This solution can then be diluted into other aqueous buffers.

Sicherheitshinweise

• Lagerklasse: 10 - Combustible liquids
• Was ist los?: WGK 3
• Flammpunkt (°F): Not applicable
• Flammpunkt (°C): Not applicable