SRP3326

BMP-2 human

Animal-component free, recombinant, expressed in E. coli, ≥98% (SDS-PAGE)

• Synonym(e): BMP-2A
• NACRES: NA.32
• UNSPSC Code: 12352202

Eigenschaften

• biological source: human
• recombinant: expressed in E. coli
• assay: ≥98% (SDS-PAGE)
• form: lyophilized
• mol wt: dimer (13.0/26.1 kDa (115/230 amino acids))
• packaging: pkg of 10 μg
• impurities: endotoxin, tested
• UniProt accession no.: P12643
• shipped in: wet ice
• storage temp.: −20°C
• Gene Information: human ... BMP2(650)

Beschreibung

General description

The functional form of BMP-2 (bone morphogenetic protein 2) is a 26kDa protein composed of two identical 114 amino-acid polypeptide chains (monomers) linked by a single disulfide bond. Each BMP-2 monomer is expressed as the C-terminal part of a precursor polypeptide, which also contains a 23 amino-acid signal sequence for secretion, and a 259 amino-acid propeptide. After dimerization of this precursor, the covalent bonds between the propeptide (which is also a disulfide-linked homodimer) and the mature BMP-2 ligand are cleaved by a furin-type protease. Recombinant human BMP-2 is a 26kDa homodimeric disulfide-linked protein consisting of two identical 115 amino acid chains.

Application

BMP-2 human has been used to study the biological role of BMP-2 on adult renal progenitor cells under in vitro and in vivo conditions.

Biochem/physiol Actions

BMP-2 (bone morphogenetic protein 2) is a potent osteoinductive cytokine, capable of inducing bone and cartilage formation in association with an osteoconductive carrier such as collagen and synthetic hydroxyapatite. During bone repair, it controls the expression of Runt-related transcription factor 2 (Runx2) and Osterix (Osx), thereby enhancing migration, proliferation and osteogenic differentiation of mesenchymal stem cells. In addition to its osteogenic activity, BMP-2 appears to play an important role in cardiac morphogenesis, and is expressed in a variety of other tissues including lung, liver, spleen, prostate, ovary, and small intestine.

Physical form

Lyophilized from a 0.2 μm filtered solution containing0.1% Trifluoroacetic Acid (TFA)

Preparation Note

Sterile water at 0.1 mg/mLCentrifuge vial before opening. Suspend the product by gently pipetting the above recommended solution down the sides of the vial. DO NOT VORTEX. Allow several minutes for complete reconstitution. For prolonged storage, dilute to working aliquots in a 0.1% BSA solution, store at -80°C, and avoid repeat freeze thaws.

Sicherheitshinweise

• Lagerklasse: 11 - Combustible Solids
• Was ist los?: WGK 3
• Flammpunkt (°F): Not applicable
• Flammpunkt (°C): Not applicable