SRP3215

JE/MCP-1 (CCL2) from mouse

recombinant, expressed in E. coli, ≥98% (SDS-PAGE), ≥98% (HPLC), suitable for cell culture

• Synonym(e): CCL2, JE, MCAF
• NACRES: NA.77
• UNSPSC Code: 12352200
• Form: lyophilized
• Assay: ≥98% (HPLC), ≥98% (SDS-PAGE)
• Biological source: mouse
• Recombinant: expressed in E. coli
• Mol wt: 13.8 kDa

Eigenschaften

• biological source: mouse
• recombinant: expressed in E. coli
• assay: ≥98% (HPLC), ≥98% (SDS-PAGE)
• form: lyophilized
• mol wt: 13.8 kDa
• packaging: pkg of 10 μg
• technique(s): cell culture | mammalian: suitable
• impurities: <0.1 EU/μg endotoxin, tested
• color: white to off-white
• UniProt accession no.: P10148
• shipped in: wet ice
• storage temp.: −20°C
• Gene Information: mouse ... CCL2(20296)

Beschreibung

General description

The monocyte chemoattractant proteins (MCPs) belong to the C-C chemokine family, and signal through C-C motif chemokine receptor-2 (CCR2) and, with the exception of MCP-1 or chemokine (C-C motif) ligand 2 (CCL2) , other CCR receptors. The MCP family cross reacts across species. The gene encoding CCL2 is localized on mouse chromosome 11. Recombinant murine JE is a 13.8kDa protein containing 125 amino acid residues including the four highly conserved cysteine residues present in the CC chemokines.

Biochem/physiol Actions

The monocyte chemoattractant proteins (MCPs) chemo attract and activate monocytes, activated T cells, basophils, natural killer (NK) cells and immature dendritic cells. It has a role in the survival and proliferation of microglia. Chemokine (C-C motif) ligand 2 (CCL2) has been shown to activate GTPases like cell division control protein 42 (CDC42) and Ras-related C3 botulinum toxin substrate 1 (Rac1).

Physical form

Lyophilized with no additives.

Preparation Note

Centrifuge the vial prior to opening. Reconstitute in water to a concentration of 0.1-1.0 mg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing a carrier protein (example 0.1% BSA) and store in working aliquots at -20°C to -80°C.

Other Notes

QPDAVNAPLT CCYSFTSKMI PMSRLESYKR ITSSRCPKEA VVFVTKLKRE VCADPKKEWV QTYIKNLDRN QMRSEPTTLF KTASALRSSA PLNVKLTRKS EANASTTFST TTSSTSVGVT SVTVN

Sicherheitshinweise

• Lagerklasse: 11 - Combustible Solids
• Was ist los?: WGK 3
• Flammpunkt (°F): Not applicable
• Flammpunkt (°C): Not applicable