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SRP3098

IL-8 (77aa) (CXCL8) human

recombinant, expressed in E. coli, ≥98% (SDS-PAGE), ≥98% (HPLC), suitable for cell culture

Synonym(e):

CXCL8, NAP-1, monocyte-derived neutrophil chemotactic factor (MDNCF), neutrophil activating factor (NAF)

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Größe/SKUVerfügbarkeitPreis
25 μg
Versand in der Regel in 2 Wochen.
€ 293,00

Über diesen Artikel

NACRES:
NA.32
UNSPSC Code:
12352202

€ 293,00


Versand in der Regel in 2 Wochen.

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biological source

human

recombinant

expressed in E. coli

assay

≥98% (HPLC), ≥98% (SDS-PAGE)

form

lyophilized

mol wt

8.9 kDa

packaging

pkg of 25 μg

technique(s)

cell culture | mammalian: suitable

impurities

<0.1 EU/μg endotoxin, tested

color

white to off-white

UniProt accession no.

shipped in

wet ice

storage temp.

−20°C

Gene Information

human ... IL8(3576)

General description

Interleukin-8 (IL-8) is a proinflammatory CXC chemokine that can signal through the C-X-C motif chemokine receptors 1 and 2 (CXCR1 and 2). It is also called as C-X-C motif chemokine ligand 8 (CXCL8). IL-8 is secreted by monocytes and endothelial cells. The gene encoding IL-8 is localized on human chromosome 4q13.3. Recombinant human IL-8 (endothelial-derived) is an 8.9kDa protein containing 77 amino acid residues.

Application

IL-8 (77aa) (CXCL8) human has been used as a G protein-coupled receptor (GPCR) agonist.

Biochem/physiol Actions

Interleukin-8 (IL-8) chemoattracts and activates neutrophils. It is involved in guiding neutrophils and oligodendrocytes to sites of infection. The protein also stimulates angiogenesis and tumor growth. It is also associated with inflammatory diseases.

Physical form

Lyophilized with no additives.

Preparation Note

Centrifuge the vial prior to opening. Reconstitute in water to a concentration of 0.1-1.0 mg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing a carrier protein (example 0.1% BSA) and store in working aliquots at -20°C to -80°C.

Other Notes

AVLPRSAKEL RCQCIKTYSK PFHPKFIKEL RVIESGPHCA NTEIIVKLSD GRELCLDPKE NWVQRVVEKF LKRAENS

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Dieser Artikel
SRP3311SRP3093MSST0057
description

recombinant, expressed in E. coli, ≥98% (SDS-PAGE), ≥98% (HPLC), suitable for cell culture

description

Animal-component free, recombinant, expressed in E. coli, ≥98% (SDS-PAGE), ≥98% (HPLC)

description

Animal-component free, recombinant, expressed in E. coli, ≥98% (SDS-PAGE), ≥98% (HPLC), suitable for cell culture

description

recombinant, expressed in HEK 293 cells, SIL MS Protein Standard, 13C and 15N-labeled

technique(s)

cell culture | mammalian: suitable

technique(s)

-

technique(s)

cell culture | mammalian: suitable

technique(s)

-

recombinant

expressed in E. coli

recombinant

expressed in E. coli

recombinant

expressed in E. coli

recombinant

expressed in HEK 293 cells

biological source

human

biological source

human

biological source

human

biological source

-

assay

≥98% (HPLC), ≥98% (SDS-PAGE)

assay

≥98% (SDS-PAGE), ≥98% (HPLC)

assay

≥98% (SDS-PAGE), ≥98% (HPLC)

assay

≥95% (SDS-PAGE)

form

lyophilized

form

lyophilized

form

lyophilized

form

lyophilized powder

mol wt

8.9 kDa

mol wt

8.4 kDa

mol wt

14.9 kDa

mol wt

-


Lagerklasse

11 - Combustible Solids

Was ist los?

WGK 3

Flammpunkt (°F)

Not applicable

Flammpunkt (°C)

Not applicable



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Questions

  1. AFTER THE INITIAL RECONSTITUTION IN WATER, CAN THIS BE DILUTED IN PBS WITH 10% FBS?

    1 answer
    1. After reconstitution with water. For extended storage, it is recommended to further dilute the solution in a buffer containing a carrier protein (example 0.1% BSA) and store it in working aliquots at -20°C to -80°C. As BSA is a significant component of FBS this would meet the long-term storage recommendation criteria.

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