SRP0726

STK21 (CIT), Active

recombinant

• NACRES: NA.77
• UNSPSC Code: 12352202
• Form: frozen liquid
• Assay: 70-95% (SDS-PAGE)
• Recombinant: expressed in baculovirus infected Sf9 cells
• Mol wt: ~74 kDa

Eigenschaften

• recombinant: expressed in baculovirus infected Sf9 cells
• assay: 70-95% (SDS-PAGE)
• form: frozen liquid
• specific activity: 4.0-6.0 nmol/min·mg (radiometric)
• mol wt: ~74 kDa
• concentration: 0.1 μg/μL
• accession no.: NM_007174
• shipped in: dry ice
• storage temp.: −70°C
• Gene Information: human ... STK21(11113)

Beschreibung

Application

Useful for kinase assay and Western blot.

Biochem/physiol Actions

Recombinant human STK21 (CIT) (1-449) was expressed by Baculovirus in Sf9 insect cells using an N-terminal GST tag. STK21 is a serine/threonine-protein kinase that functions in cell division and also to promote efficient cytokinesis. STK21 is a novel molecular mechanism for a subset of human malformation syndromes of the central nervous system ⁽¹⁾. STK21 is also associated with bipolar disorder and risk for schizophrenia. STK21 is a novel partner for the GTP-bound forms of rho and rac ⁽²⁾.

Preparation Note

Formulated in 50mM Tris-HCl, pH 7.5, 150mM NaCl, 10mM glutathione, 0.1mM EDTA, 0.25 mM DTT, 0.1 mM PMSF, with 25% glycerol.

Store in aliquots at -70°C. Avoid freeze/thaw cycles.

Sicherheitshinweise

• Lagerklasse: 10 - Combustible liquids
• wgk: WGK 1
• flash_point_f: Not applicable
• flash_point_c: Not applicable