SRP0243

aRAF Active human

recombinant, expressed in baculovirus infected insect cells, ≥80% (SDS-PAGE)

• Synonym(e): A-Raf proto-oncogene, PKS2, v-Raf murine sarcoma 3611 viral oncogene homolog
• UNSPSC-Code: 12352202
• NACRES: NA.32

Eigenschaften

• Biologische Quelle: human
• Rekombinant: expressed in baculovirus infected insect cells
• Assay: ≥80% (SDS-PAGE)
• Form: aqueous solution
• Mol-Gew.: 63.6 kDa
• Verpackung: pkg of 10 μg
• Lagerbedingungen: avoid repeated freeze/thaw cycles
• Konzentration: >0.02 mg/mL
• NCBI-Hinterlegungsnummer: NM_001654
• UniProt-Hinterlegungsnummer: P10398
• Versandbedingung: dry ice
• Lagertemp.: −70°C
• Angaben zum Gen: human ... ARAF(369)

Beschreibung

Allgemeine Beschreibung

Human A-RAF (GenBank Accession No. NM_001654), amino acids 282-end, containing the mutations YY301-302DD, MW = 63.6 kDa, with N-terminal GST tag, expressed in an Baculovirus infected Sf9 cell expression system.

Anwendung

Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.

Physikalische Form

Formulated in 25 mM Tris-HCl, pH 8.0, 100 mM NaCl, 0.05% Tween-20, 50% glycerol, and 3 mM DTT.

Angaben zur Herstellung

Thaw on ice. Upon first thaw, briefly spin tube containing enzyme to recover full content of the tube. Aliquot enzyme into single use aliquots. Store remaining undiluted enzyme in aliquots at -70°C. Note: Enzyme is very sensitive to freeze/thaw cycles.