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SHC332V
MISSION® 3X LacO Inducible Non-Target shRNA Control Transduction Particles
Does not target any known genes
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Über diesen Artikel
NACRES:
NA.51
UNSPSC Code:
41106609
Concentration:
≥1x106 VP/ml (via p24 assay)
Preise und Verfügbarkeit sind derzeit nicht verfügbar.
Technischer Dienst
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Unterstützung erhaltenGeneral description
Sigma® is proud to offer IPTG-inducible vectors as the latest development from our continued partnership in TRC.
The pLKO vector has been redesigned to contain a LacI (repressor) and a modified human U6 shRNA promoter with LacO (operator) sequences. In the absence of IPTG (isopropyl-Β-D-thio-galactoside), an analogue of lactose, LacI binds to LacO preventing expression of the shRNA. When IPTG is present, the allosteric LacI repressor changes conformation, releasing itself from lacO modified human U6 promoter, and subsequently allows expression of the shRNA.
The MISSION 3x LacO Inducible Non-Target shRNA Control Transduction Particles contain an shRNA insert that does not target any known genes, making it useful as a negative control in experiments using the MISSION inducible shRNA library clones. This allows one to examine the effect of transfection of a short-hairpin on gene expression and interpret the knockdown effect seen with shRNA clones. Ampicillin and puromycin antibiotic resistance genes provide selection in bacterial or mammalian cells respectively. In addition, self-inactivating replication incompetent viral particles can be produced in packaging cells (HEK293T) by co-transfection with compatible packaging plasmids. 200 uL of 106 TU/ml (via p24 titering assay) lentiviral particles are provided as frozen stock.
When conducting experiments using MISSION® shRNA clones, the proper controls should be a key element of your experimental design to allow for accurate interpretation of knockdown results.
The pLKO vector has been redesigned to contain a LacI (repressor) and a modified human U6 shRNA promoter with LacO (operator) sequences. In the absence of IPTG (isopropyl-Β-D-thio-galactoside), an analogue of lactose, LacI binds to LacO preventing expression of the shRNA. When IPTG is present, the allosteric LacI repressor changes conformation, releasing itself from lacO modified human U6 promoter, and subsequently allows expression of the shRNA.
The MISSION 3x LacO Inducible Non-Target shRNA Control Transduction Particles contain an shRNA insert that does not target any known genes, making it useful as a negative control in experiments using the MISSION inducible shRNA library clones. This allows one to examine the effect of transfection of a short-hairpin on gene expression and interpret the knockdown effect seen with shRNA clones. Ampicillin and puromycin antibiotic resistance genes provide selection in bacterial or mammalian cells respectively. In addition, self-inactivating replication incompetent viral particles can be produced in packaging cells (HEK293T) by co-transfection with compatible packaging plasmids. 200 uL of 106 TU/ml (via p24 titering assay) lentiviral particles are provided as frozen stock.
When conducting experiments using MISSION® shRNA clones, the proper controls should be a key element of your experimental design to allow for accurate interpretation of knockdown results.
Application
To see more application data, protocols, vector maps visit sigma.com/shrna.
Legal Information
MISSION is a registered trademark of Merck KGaA, Darmstadt, Germany
Sigma is a registered trademark of Sigma-Aldrich Co. LLC
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Dieser Artikel | |||
|---|---|---|---|
| description Does not target any known genes | description Does not target any known genes | description Targets no known mammalian genes | description Targets no known genes from any species |
| concentration ≥1x106 VP/ml (via p24 assay) | concentration 500 ng/μL in TE buffer; DNA (10μg of plasmid DNA) | concentration ≥1x106 VP/ml (via p24 assay) | concentration ≥1x106 VP/ml (via p24 assay) |
| product line MISSION® | product line MISSION® | product line MISSION® | product line MISSION® |
| Quality Level 100 | Quality Level 200 | Quality Level 100 | Quality Level 100 |
Lagerklasse
12 - Non Combustible Liquids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
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